Hybridization and Spore Dissection of Native Wine Yeasts for Improvement of Ethanol Resistance and Osmotolerance

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2022 Sep 19

PMID 36121519

Authors

Maria Laura Sanchez

Selva Valeria Chimeno

Laura Analia Mercado

Ivan Francisco Ciklic

Affiliations

Soon will be listed here.

Abstract

Global warming has a significant impact on different viticultural parameters, including grape maturation. An increment of photosynthetic activity generates a rapid accumulation of sugars in the berry, followed by a dehydration process which leads to a higher concentration of soluble solids. This effect is exacerbated by current viticultural practices which favor the harvest of very mature grapes to obtain wines with sweet tannins. Considering the initial hyperosmotic stress conditions and the high ethanol concentration of the produced wine, fermentation of grape musts with high sugar content could be problematic for yeast starters. In the present study, we were able to obtain by classical hybridization and spore dissection methods one hybrid and one monosporic wine yeast strain with a combined ethanol and osmotolerant phenotype. The improved yeasts were tested in vinification trials with high sugar concentration and displayed excellent fermentation performance. Importantly, the obtained wines also showed good organoleptic properties during sensory analysis. Based on our results, we believed our improved hybrid and monosporic strains can be considered good alternatives to be used as yeast starters for fermentations with high sugar content.

References

Bonciani T, De Vero L, Mezzetti F, Fay J, Giudici P . A multi-phase approach to select new wine yeast strains with enhanced fermentative fitness and glutathione production. Appl Microbiol Biotechnol. 2018; 102(5):2269-2278. DOI: 10.1007/s00253-018-8773-3. View

Boveri S, Rainieri S, Pulvirenti A . Method for the validation of intraspecific crosses of Saccharomyces cerevisiae strains by minisatellite analysis. Can J Microbiol. 2012; 58(3):350-8. DOI: 10.1139/w11-142. View

Cakar Z, Seker U, Tamerler C, Sonderegger M, Sauer U . Evolutionary engineering of multiple-stress resistant Saccharomyces cerevisiae. FEMS Yeast Res. 2005; 5(6-7):569-78. DOI: 10.1016/j.femsyr.2004.10.010. View

Caridi A, Sidari R, Solieri L, Cufari A, Giudici P . Wine colour adsorption phenotype: an inheritable quantitative trait loci of yeasts. J Appl Microbiol. 2007; 103(3):735-42. DOI: 10.1111/j.1365-2672.2007.03301.x. View

Clavijo A, Calderon I, Paneque P . Effect of the use of commercial Saccharomyces strains in a newly established winery in Ronda (Málaga, Spain). Antonie Van Leeuwenhoek. 2010; 99(3):727-31. DOI: 10.1007/s10482-010-9514-5. View

DAlessio C, Caramelo J, Parodi A . Absence of nucleoside diphosphatase activities in the yeast secretory pathway does not abolish nucleotide sugar-dependent protein glycosylation. J Biol Chem. 2005; 280(49):40417-27. DOI: 10.1074/jbc.M503149200. View

Donalies U, Nguyen H, Stahl U, Nevoigt E . Improvement of Saccharomyces yeast strains used in brewing, wine making and baking. Adv Biochem Eng Biotechnol. 2008; 111:67-98. DOI: 10.1007/10_2008_099. View

Fleet G . Wine yeasts for the future. FEMS Yeast Res. 2008; 8(7):979-95. DOI: 10.1111/j.1567-1364.2008.00427.x. View

Garcia V, Rivera J, Contreras A, Ganga M, Martinez C . Development and characterization of hybrids from native wine yeasts. Braz J Microbiol. 2013; 43(2):482-9. PMC: 3768851. DOI: 10.1590/S1517-83822012000200008. View

10.

Garcia-Rios E, Guillen A, De La Cerda R, Perez-Traves L, Querol A, Guillamon J . Improving the Cryotolerance of Wine Yeast by Interspecific Hybridization in the Genus . Front Microbiol. 2019; 9:3232. PMC: 6331415. DOI: 10.3389/fmicb.2018.03232. View

11.

Giudici P, Solieri L, Pulvirenti A, Cassanelli S . Strategies and perspectives for genetic improvement of wine yeasts. Appl Microbiol Biotechnol. 2004; 66(6):622-8. DOI: 10.1007/s00253-004-1784-2. View

12.

Hoffman C, Winston F . A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene. 1987; 57(2-3):267-72. DOI: 10.1016/0378-1119(87)90131-4. View

13.

Jara C, Laurie V, Mas A, Romero J . Microbial Terroir in Chilean Valleys: Diversity of Non-conventional Yeast. Front Microbiol. 2016; 7:663. PMC: 4868835. DOI: 10.3389/fmicb.2016.00663. View

14.

Legras J, Karst F . Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation. FEMS Microbiol Lett. 2003; 221(2):249-55. DOI: 10.1016/S0378-1097(03)00205-2. View

15.

Lopes C, van Broock M, Querol A, Caballero A . Saccharomyces cerevisiae wine yeast populations in a cold region in Argentinean Patagonia. A study at different fermentation scales. J Appl Microbiol. 2002; 93(4):608-15. DOI: 10.1046/j.1365-2672.2002.01738.x. View

16.

Lopes C, Rodriguez M, Sangorrin M, Querol A, Caballero A . Patagonian wines: the selection of an indigenous yeast starter. J Ind Microbiol Biotechnol. 2007; 34(8):539-46. DOI: 10.1007/s10295-007-0227-3. View

17.

Marullo P, Bely M, Masneuf-Pomarede I, Aigle M, Dubourdieu D . Inheritable nature of enological quantitative traits is demonstrated by meiotic segregation of industrial wine yeast strains. FEMS Yeast Res. 2004; 4(7):711-9. DOI: 10.1016/j.femsyr.2004.01.006. View

18.

Marullo P, Bely M, Masneuf-Pomarede I, Pons M, Aigle M, Dubourdieu D . Breeding strategies for combining fermentative qualities and reducing off-flavor production in a wine yeast model. FEMS Yeast Res. 2006; 6(2):268-79. DOI: 10.1111/j.1567-1364.2006.00034.x. View

19.

Perez-Traves L, Lopes C, Barrio E, Querol A . Evaluation of different genetic procedures for the generation of artificial hybrids in Saccharomyces genus for winemaking. Int J Food Microbiol. 2012; 156(2):102-11. DOI: 10.1016/j.ijfoodmicro.2012.03.008. View

20.

Steensels J, Snoek T, Meersman E, Picca Nicolino M, Voordeckers K, Verstrepen K . Improving industrial yeast strains: exploiting natural and artificial diversity. FEMS Microbiol Rev. 2014; 38(5):947-95. PMC: 4293462. DOI: 10.1111/1574-6976.12073. View