Mechanism of Huoxue Tongluo Decoction in Treatment of Erectile Dysfunction Caused by Ischemic Stroke Based on Network Pharmacology

Overview

Journal Chin Herb Med

Publisher Elsevier

Date 2022 Sep 19

PMID 36118932

Authors

Ji-Sheng Wang

Heng-Heng Dai

Kai-Ge Zhang

Ke-Gang Cao

Sheng Deng

Bing-Hao Bao

Jun-Long Feng

Fan-Chao Meng

Hai-Song Li

Bin Wang

Affiliations

Soon will be listed here.

Abstract

Objective: To study the therapeutic effect of Huoxue Tongluo Decoction (HXTLD) on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.

Methods: Network pharmacology was used to predict the key active ingredients and targets of HXTLD. Surgical methods were used to create a rat model of ischemic stroke. The rats were then given a suspension of HXTLD by ig administration. Erectile function was evaluated by Apomorphine (APO) induction. Real-time fluorescence quantitative reverse transcription-polymerase chain reaction (Real-time PCR) and Western blotting were used to detect the expression of related mRNAs and proteins in rat penile corpus cavernous tissue and brain tissue. Hematoxylin & Eosin (HE) staining was used to investigate structural changes in the penile cavernous tissue.

Results: Network pharmacology showed that tumor necrosis factor (TNF), nitric oxide synthase 3 (eNOS), and vascular endothelial growth factor (VEGF) were the key targets of HXTLD in the treatment of erectile dysfunction caused by ischemic stroke. Experimental studies showed that HXTLD improved erectile dysfunction caused by ischemic stroke. HE results showed that HXTLD improved the structure of the corpus cavernosa. HXTLD also inhibited the expression of TNF and VEGF proteins in penile tissue ( < 0.05) and enhanced the expression of eNOS protein in penile tissue ( < 0.05).

Conclusion: HXTLD improved the erectile function of rats with erectile dysfunction caused by ischemic stroke by regulating the mRNA and protein levels of TNF, eNOS and VEGF.

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