Transcriptome Analysis Reveals the Mechanism of Chronic Heat Stress on Meat Quality of Broilers

Overview

Journal J Anim Sci Biotechnol

Publisher Biomed Central

Date 2022 Sep 18

PMID 36117193

Authors

Zhen Liu

Yingsen Liu

Tong Xing

Jiaolong Li

Lin Zhang

Yun Jiang

Feng Gao

Affiliations

Soon will be listed here.

Abstract

Background: Chronic heat stress has a negative impact on poultry meat quality. Although this has been extensively investigated, previous studies have primarily focused on metabolic alterations and oxidative stress in the pectoralis major (PM) muscle under chronic heat stress, and not all of the underlying molecular mechanisms are completely understood.

Methods: A total of 144 male Arbor Acres broilers (28 d old) were randomly allocated into 3 treatment groups: (1) the normal control (NC) group, with broilers raised at 22 °C and fed a basal diet; (2) the heat stress (HS) group, with birds raised at 32 °C and fed a basal diet; and (3) the pair-fed (PF) group, with birds raised at 22 °C and fed the amount of feed equal to the feed consumed on the previous day by the HS group. The experiment lasted for 14 d.

Results: Chronic heat stress decreased the average daily feed intake and average daily gain, increased feed:gain ratio (P < 0.05); and increased drip loss, cooking loss, shear force, hardness, and decreased pH, redness (a); and springiness of PM muscle (P < 0.05). Furthermore, chronic heat stress decreased muscle fiber density, increased connective tissue, and led to intracellular vacuolation. The transcriptome analyses indicated that the effect of chronic heat stress on meat quality was not only related to metabolism and oxidative stress, but also to signal transduction, immune system, transport and catabolism, cell growth and death, and muscle structure.

Conclusions: Chronic heat stress has a negative impact on the growth performance, meat quality, and the PM muscle structure of broilers. Transcriptome analysis revealed a comprehensive understanding of the mechanism of the chronic heat stress-induced deterioration of broiler meat quality at the transcriptional level.

Citing Articles

Applications of Next-Generation Sequencing Technologies and Statistical Tools in Identifying Pathways and Biomarkers for Heat Tolerance in Livestock.

Kalaignazhal G, Sejian V, Velayudhan S, Mishra C, Rebez E, Chauhan S Vet Sci. 2024; 11(12).

PMID: 39728955 PMC: 11680151. DOI: 10.3390/vetsci11120616.

Comparison of High n-3 PUFA Levels and Cyclic Heat Stress Effects on Carcass Characteristics, Meat Quality, and Oxidative Stability of Breast Meat of Broilers Fed Low- and High-Antioxidant Diets.

Pecjak Pal M, Leskovec J, Levart A, Pirman T, Salobir J, Rezar V Animals (Basel). 2024; 14(22).

PMID: 39595366 PMC: 11591467. DOI: 10.3390/ani14223314.

Unraveling the role of long non-coding RNAs in chronic heat stress-induced muscle injury in broilers.

Liu Z, Liu Y, Xing T, Li J, Zhang L, Zhao L J Anim Sci Biotechnol. 2024; 15(1):135.

PMID: 39375773 PMC: 11459952. DOI: 10.1186/s40104-024-01093-6.

Dietary Organic Zinc Supplementation Modifies the Oxidative Genes via RORγ and Epigenetic Regulations in the Ileum of Broiler Chickens Exposed to High-Temperature Stress.

Adam S, Muniyappan M, Huang H, Ennab W, Liu H, Ahmed A Antioxidants (Basel). 2024; 13(9).

PMID: 39334738 PMC: 11429418. DOI: 10.3390/antiox13091079.

Nano-Se exhibits limited protective effect against heat stress induced poor breast muscle meat quality of broilers compared with other selenium sources.

Jing J, Wang J, Wu Q, Yin S, He Z, Tang J J Anim Sci Biotechnol. 2024; 15(1):95.

PMID: 38972969 PMC: 11229195. DOI: 10.1186/s40104-024-01051-2.

References

Ganesan S, Pearce S, Gabler N, Baumgard L, Rhoads R, Selsby J . Short-term heat stress results in increased apoptotic signaling and autophagy in oxidative skeletal muscle in Sus scrofa. J Therm Biol. 2018; 72:73-80. DOI: 10.1016/j.jtherbio.2018.01.003. View

Zhao L, McMillan R, Xie G, Giridhar S, Baumgard L, El-Kadi S . Heat stress decreases metabolic flexibility in skeletal muscle of growing pigs. Am J Physiol Regul Integr Comp Physiol. 2018; 315(6):R1096-R1106. DOI: 10.1152/ajpregu.00404.2017. View

Abudabos A, Suliman G, Al-Owaimer A, Al Sulaiman A, Alharthi A . Effects of Nano Emulsified Vegetable Oil and Betaine on Growth Traits and Meat Characteristics of Broiler Chickens Reared under Cyclic Heat Stress. Animals (Basel). 2021; 11(7). PMC: 8300220. DOI: 10.3390/ani11071911. View

Wang S, Li X, Wang W, Zhang H, Xu S . Application of transcriptome analysis: Oxidative stress, inflammation and microtubule activity disorder caused by ammonia exposure may be the primary factors of intestinal microvilli deficiency in chicken. Sci Total Environ. 2019; 696:134035. DOI: 10.1016/j.scitotenv.2019.134035. View

Xie C, Mao X, Huang J, Ding Y, Wu J, Dong S . KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases. Nucleic Acids Res. 2011; 39(Web Server issue):W316-22. PMC: 3125809. DOI: 10.1093/nar/gkr483. View

Noeman S, Hamooda H, Baalash A . Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetol Metab Syndr. 2011; 3(1):17. PMC: 3174870. DOI: 10.1186/1758-5996-3-17. View

Squire J . Special Issue: The Actin-Myosin Interaction in Muscle: Background and Overview. Int J Mol Sci. 2019; 20(22). PMC: 6887992. DOI: 10.3390/ijms20225715. View

Scheffler T, Gerrard D . Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Sci. 2011; 77(1):7-16. DOI: 10.1016/j.meatsci.2007.04.024. View

Perini F, Cendron F, Rovelli G, Castellini C, Cassandro M, Lasagna E . Emerging Genetic Tools to Investigate Molecular Pathways Related to Heat Stress in Chickens: A Review. Animals (Basel). 2021; 11(1). PMC: 7823582. DOI: 10.3390/ani11010046. View

10.

Xu Y, Lai X, Li Z, Zhang X, Luo Q . Effect of chronic heat stress on some physiological and immunological parameters in different breed of broilers. Poult Sci. 2018; 97(11):4073-4082. PMC: 6162357. DOI: 10.3382/ps/pey256. View

11.

Bernabucci U, Lacetera N, Baumgard L, Rhoads R, Ronchi B, Nardone A . Metabolic and hormonal acclimation to heat stress in domesticated ruminants. Animal. 2012; 4(7):1167-83. DOI: 10.1017/S175173111000090X. View

12.

Chen X, Zhang L, Li J, Gao F, Zhou G . Hydrogen Peroxide-Induced Change in Meat Quality of the Breast Muscle of Broilers Is Mediated by ROS Generation, Apoptosis, and Autophagy in the NF-κB Signal Pathway. J Agric Food Chem. 2017; 65(19):3986-3994. DOI: 10.1021/acs.jafc.7b01267. View

13.

Sula M, Winslow C, Boileau M, Barker L, Panciera R . Heat-related injury in lambs. J Vet Diagn Invest. 2012; 24(4):772-6. DOI: 10.1177/1040638712445772. View

14.

Cui Y, Zhou X, Chen L, Tang Z, Mo F, Li X . Crosstalk between Endoplasmic Reticulum Stress and Oxidative Stress in Heat Exposure-Induced Apoptosis Is Dependent on the ATF4-CHOP-CHAC1 Signal Pathway in IPEC-J2 Cells. J Agric Food Chem. 2021; 69(51):15495-15511. DOI: 10.1021/acs.jafc.1c03361. View

15.

Li G, Liu L, Yin B, Liu Y, Dong W, Gong S . Heat stress decreases egg production of laying hens by inducing apoptosis of follicular cells via activating the FasL/Fas and TNF-α systems. Poult Sci. 2020; 99(11):6084-6093. PMC: 7647730. DOI: 10.1016/j.psj.2020.07.024. View

16.

Lu Z, He X, Ma B, Zhang L, Li J, Jiang Y . The alleviative effects and related mechanisms of taurine supplementation on growth performance and carcass characteristics in broilers exposed to chronic heat stress. Poult Sci. 2018; 98(2):878-886. DOI: 10.3382/ps/pey433. View

17.

Lu Z, He X, Ma B, Zhang L, Li J, Jiang Y . Chronic Heat Stress Impairs the Quality of Breast-Muscle Meat in Broilers by Affecting Redox Status and Energy-Substance Metabolism. J Agric Food Chem. 2017; 65(51):11251-11258. DOI: 10.1021/acs.jafc.7b04428. View

18.

Chen Y, Yu T . Mouse liver is more resistant than skeletal muscle to heat-induced apoptosis. Cell Stress Chaperones. 2020; 26(1):275-281. PMC: 7736438. DOI: 10.1007/s12192-020-01163-4. View

19.

Zaboli G, Huang X, Feng X, Ahn D . How can heat stress affect chicken meat quality? - a review. Poult Sci. 2018; 98(3):1551-1556. DOI: 10.3382/ps/pey399. View

20.

Lu Q, Wen J, Zhang H . Effect of chronic heat exposure on fat deposition and meat quality in two genetic types of chicken. Poult Sci. 2007; 86(6):1059-64. DOI: 10.1093/ps/86.6.1059. View