Epigenetic Dynamics of H4K20me3 Modification During Oocyte Maturation and Early Reprogramming of Somatic Cell Nuclear Transfer Goat Embryos

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2022 Sep 15

PMID 36105059

Authors

Zhihui Liu

Mingyang Li

Yu Sun

Weiguo Wang

Zhisong Wang

Giorgio Antonio Presicce

Liyou An

Fuliang Du

Affiliations

Soon will be listed here.

Abstract

Objective: We examined the epigenetic dynamics of histone H4K20 trimethylation (H4K20me3), a repressive signature in heterochromatin, during goat oocyte meiosis and the reprogramming of somatic cell nuclear transfer (NT) embryos through the first three cell divisions.

Methods: Following NT, oocytes were treated with parthenogenetic activation (PA), by 5 µM calcium ionophore A23187 for 5 min followed by incubation in 2.0 mM 6-dimethylaminopurine with 5 µg/mL cycloheximide for 4 h. NT embryos up to 8-celled stage were incubated with H4K20me3 antibody.

Results: Immunofluorescence microscopy revealed the existence of a persistent H4K20me3 signature during oocyte maturation from germinal vesicle phase to metaphase I, anaphase I, telophase I, and metaphase II, with a gradual reduction in staining intensity. NT embryos at the 2-, 4- and 8-celled stage showed lower H4K20me3 intensity than PA and IVF embryos ( < 0.05).

Conclusion: These results indicate that NT embryos exhibit insufficient H4K20me3 modification compared with IVF and PA embryos during early reprogramming, suggesting the existence of a resistant memory of differentiated cell nuclear architecture. These findings help unravel the epigenetic mechanism of histone H4K20me3 in goat nuclear transfer reprogramming.

Citing Articles

Overcoming the H4K20me3 epigenetic barrier improves somatic cell nuclear transfer reprogramming efficiency in mice.

Liu Z, Wang W, Xia Y, Gao Y, Wang Z, Li M Cell Prolif. 2023; 57(1):e13519.

PMID: 37322828 PMC: 10771106. DOI: 10.1111/cpr.13519.

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