Transcriptional Regulation of Proanthocyanidin Biosynthesis Pathway Genes and Transcription Factors in Indigofera Stachyodes Lindl. Roots

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2022 Sep 12

PMID 36096752

Authors

Chongmin Wang

Jun Li

Tao Zhou

YongPing Zhang

Haijun Jin

Xiaoqing Liu

Affiliations

Soon will be listed here.

Abstract

Background: Proanthocyanidins (PAs) have always been considered as important medicinal value component. In order to gain insights into the PA biosynthesis regulatory network in I. stachyodes roots, we analyzed the transcriptome of the I. stachyodes in Leaf, Stem, RootI (one-year-old root), and RootII (two-year-old root).

Results: In this study, a total of 110,779 non-redundant unigenes were obtained, of which 63,863 could be functionally annotated. Simultaneously, 75 structural genes that regulate PA biosynthesis were identified, of these 6 structural genes (IsF3'H1, IsANR2, IsLAR2, IsUGT72L1-3, IsMATE2, IsMATE3) may play an important role in the synthesis of PAs in I. stachyodes roots. Furthermore, co-expression network analysis revealed that 34 IsMYBs, 18 IsbHLHs, 15 IsWRKYs, 9 IsMADSs, and 3 IsWIPs hub TFs are potential regulators for PA accumulation. Among them, IsMYB24 and IsMYB79 may be closely involved in the PA biosynthesis in I. stachyodes roots.

Conclusions: The biosynthesis of PAs in I. stachyodes roots is mainly produced by the subsequent pathway of cyanidin. Our work provides new insights into the molecular pathways underlying PA accumulation and enhances our global understanding of transcriptome dynamics throughout different tissues.

Citing Articles

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Wang C, Yang J, Pan Q, Zhu P, Li J Front Nutr. 2024; 11:1417526.

PMID: 39036490 PMC: 11258014. DOI: 10.3389/fnut.2024.1417526.

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