Super-resolution of the Brain - How to Visualize the Hidden Nanoplasticity?

Overview

Journal iScience

Publisher Cell Press

Date 2022 Sep 12

PMID 36093060

Authors

Katrin I Willig

Affiliations

Soon will be listed here.

Abstract

Super-resolution fluorescence microscopy has entered most biological laboratories worldwide and its benefit is undisputable. Its application to brain imaging, for example in living mice, enables the study of sub-cellular structural plasticity and brain function directly in a living mammal. The demands of brain imaging on the different super-resolution microscopy techniques (STED, RESOLFT, SIM, ISM) and labeling strategies are discussed here as well as the challenges of the required cranial window preparation. Applications of super-resolution in the anesthetized mouse brain enlighten the stability and plasticity of synaptic nanostructures. These studies show the potential of super-resolution imaging and justify its application more widely to investigate the role of nanostructures in memory and learning.

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