Genome-wide Association Study for Grain Yield and Component Traits in Bread Wheat ( L.)

Overview

Journal Front Genet

Date 2022 Sep 12

PMID 36092913

Authors

Hanif Khan

Gopalareddy Krishnappa

Satish Kumar

Chandra Nath Mishra

Hari Krishna

Narayana Bhat Devate

Nagenahalli Dharmegowda Rathan

Om Parkash

Sonu Singh Yadav

Puja Srivastava

Suma Biradar

Monu Kumar

Gyanendra Pratap Singh

Affiliations

Soon will be listed here.

Abstract

Genomic regions governing days to heading (DH), grain filling duration (GFD), grain number per spike (GNPS), grain weight per spike (GWPS), plant height (PH), and grain yield (GY) were investigated in a set of 280 diverse bread wheat genotypes. The genome-wide association studies (GWAS) panel was genotyped using a 35K Axiom Array and phenotyped in five environments. The GWAS analysis showed a total of 27 Bonferroni-corrected marker-trait associations (MTAs) on 15 chromosomes representing all three wheat subgenomes. The GFD showed the highest MTAs (8), followed by GWPS (7), GY (4), GNPS (3), PH (3), and DH (2). Furthermore, 20 MTAs were identified with more than 10% phenotypic variation. A total of five stable MTAs (, and ) were identified in more than one environment and associated with the expression of DH, GFD, GNPS, and GY. Similarly, two novel pleiotropic genomic regions with associated MTAs i.e. (4D) and (6A) harboring co-localized QTLs governing two or more traits were also identified. analysis revealed that the SNPs were located on important putative candidate genes such as , , , , involved in the regulation of grain size, grain number, growth and development, grain filling duration, and abiotic stress tolerance. The identified novel MTAs will be validated to estimate their effects in different genetic backgrounds for subsequent use in marker-assisted selection (MAS).

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