Interleukin 13 Participates in Terminal Differentiation of Esophageal Squamous Cell Carcinoma Cells

Overview

Journal J Gastrointest Oncol

Date 2022 Sep 12

PMID 36092316

Authors

Jian Li

Wenjian Wang

Ke Wang

Guangwei Ma

Jing Shao

Weizhen Fang

Yiting Zhou

Jiatong Lin

Yabin Guo

Xinyuan Guan

Chaohui Duan

Affiliations

Soon will be listed here.

Abstract

Background: In China, esophageal squamous cell carcinoma (ESCC) accounts for more than 90% of all esophageal cancer cases. Interleukin 13 (IL-13) was widely reported to play a key role in tumor progression. Our previous study reported that IL-13 was a favorable predictive marker for the overall survival of esophageal squamous cell carcinoma (ESCC) patients, but how IL-13 contributes to ESCC progression remains unknown. This study aims to explore the role of IL-13 and its underlying downstream molecular mechanisms in ESCC progression.

Methods: Tissue microarrays including 262 primary ESCC tumor tissues were collected and analyzed. The expression of IL-13 in ESCC tumor tissue was detected with immunohistochemistry staining (IHC). Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to qualify the expressions of , and 15-lipoxygenase-1 () in cultured ESCC cell lines with recombinant IL-13 treatment.

Results: IL-13 was expressed in the esophageal epithelium cells and ESCC tumor cells. High IL-13 expression in ESCC tumor cells predicted a good prognosis for patients. Recombinant human IL-13 raised and mRNA levels, but lowered mRNA level 15-LOX-1 in ESCC cells .

Conclusions: In summary, our study suggests that IL-13 might improve the prognosis of ESCC by promoting the terminal differentiation of ESCC cells. This may offer potential new therapeutic target for early treatment of ESCC.

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