Application of Metabolomics and Network Analysis to Reveal the Ameliorating Effect of Four Typical "hot" Property Herbs on Hypothyroidism Rats

Overview

Journal Front Pharmacol

Date 2022 Sep 12

PMID 36091783

Authors

Yang-Yang Wang

Yan-Ping Sun

Bing-You Yang

Qiu-Hong Wang

Hai-Xue Kuang

Affiliations

Soon will be listed here.

Abstract

Herbs with a "hot" properties are frequently used to treat cold symptoms in TCM. However, the underlying mechanisms of the herbs with "hot" properties on hypothyroidism have not been investigated. This study aimed to explore four typical "hot" and "cold" property herb on hypothyroidism. Firstly, the difference efficacy between the four typical "hot" property herbs and the four typical "cold" property herbs was assessed by physical signs, thyroid function, and the metabolic profile using multivariate statistical analysis. The influence of the four typical "hot" property herbs on hypothyroidism was validated pathologically. The impact mechanism of the four typical "hot" property herbs on hypothyroidism was investigated through a metabolomics method combined with network analysis. Na/K-ATP, ACC1 enzyme, UCP-1, and the PI3K-Akt pathway were used to confirm the metabolite pathways and target-associated metabolites. The results showed that the four typical "hot" property herbs could significantly improve physical signs, thyroid function, and the metabolic profile in hypothyroidism rats, the four typical "cold" property herbs did not show any benefit. Moreover, the four typical "hot" property herbs could improve lipid metabolism, energy metabolism, and thyroid hormone levels by the PI3K-Akt signaling pathway, Ca- AMPK signaling pathways, purine metabolism, and tryptophan metabolism. Additionally, the levels of UCP-1, Na+/K + -ATP enzyme, and ACC1 were ameliorated by the four typical "hot" property herbs in hypothyroidism rats. Therefore, a metabolomics strategy combined with network analysis was successfully performed and interpreted the mechanism of the four typical "hot" property herbs on hypothyroidism based on the theory of "cold and hot" properties of TCM well.

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References

Flynn R, Bonellie S, Jung R, MacDonald T, Morris A, Leese G . Serum thyroid-stimulating hormone concentration and morbidity from cardiovascular disease and fractures in patients on long-term thyroxine therapy. J Clin Endocrinol Metab. 2009; 95(1):186-93. DOI: 10.1210/jc.2009-1625. View

Pan L, Li Z, Wang Y, Zhang B, Liu G, Liu J . Network pharmacology and metabolomics study on the intervention of traditional Chinese medicine Huanglian Decoction in rats with type 2 diabetes mellitus. J Ethnopharmacol. 2020; 258:112842. DOI: 10.1016/j.jep.2020.112842. View

Ahmed O, Abd El-Tawab S, Ahmed R . Effects of experimentally induced maternal hypothyroidism and hyperthyroidism on the development of rat offspring: I. The development of the thyroid hormones-neurotransmitters and adenosinergic system interactions. Int J Dev Neurosci. 2010; 28(6):437-54. DOI: 10.1016/j.ijdevneu.2010.06.007. View

Feng Y, Wu Z, Zhou X, Zhou Z, Fan W . Knowledge discovery in traditional Chinese medicine: state of the art and perspectives. Artif Intell Med. 2006; 38(3):219-36. DOI: 10.1016/j.artmed.2006.07.005. View

Cheng X, Wei Z, Zhang G, Shao X, Li B, Gao R . A systematic review of combinatorial treatment with warming and invigorating drugs and levothyroxine for hypothyroidism caused by Hashimoto disease. Ann Transl Med. 2017; 4(23):459. PMC: 5220024. DOI: 10.21037/atm.2016.12.16. View

Krotkiewski M . Thyroid hormones in the pathogenesis and treatment of obesity. Eur J Pharmacol. 2002; 440(2-3):85-98. DOI: 10.1016/s0014-2999(02)01420-6. View

Friedrich N, Pietzner M, Cannet C, Thuesen B, Hansen T, Wallaschofski H . Urinary metabolomics reveals glycemic and coffee associated signatures of thyroid function in two population-based cohorts. PLoS One. 2017; 12(3):e0173078. PMC: 5333857. DOI: 10.1371/journal.pone.0173078. View

Ren X, Zhang J, Fu X, Ma S, Wang C, Wang J . LC-MS based metabolomics identification of novel biomarkers of tobacco smoke-induced chronic bronchitis. Biomed Chromatogr. 2015; 30(1):68-74. DOI: 10.1002/bmc.3620. View

Li F, Guo S, Zhan T, Mo H, Chen X, Wang H . Integrating network pharmacology and experimental evidence to decipher the cardioprotective mechanism of Yiqihuoxue decoction in rats after myocardial infarction. J Ethnopharmacol. 2021; 279:114062. DOI: 10.1016/j.jep.2021.114062. View

10.

Vaccari A, Rossetti Z, de Montis G, Stefanini E, Martino E, Gessa G . Neonatal hypothyroidism induces striatal dopaminergic dysfunction. Neuroscience. 1990; 35(3):699-706. DOI: 10.1016/0306-4522(90)90340-a. View

11.

Clarke N, Kabadi U . Optimizing treatment of hypothyroidism. Treat Endocrinol. 2005; 3(4):217-21. DOI: 10.2165/00024677-200403040-00003. View

12.

Beckett G, Kellett H, Gow S, Hussey A, Hayes J, Toft A . Raised plasma glutathione S-transferase values in hyperthyroidism and in hypothyroid patients receiving thyroxine replacement: evidence for hepatic damage. Br Med J (Clin Res Ed). 1985; 291(6493):427-31. PMC: 1416337. DOI: 10.1136/bmj.291.6493.427. View

13.

Wu S, Tan G, Dong X, Zhu Z, Li W, Lou Z . Metabolic profiling provides a system understanding of hypothyroidism in rats and its application. PLoS One. 2013; 8(2):e55599. PMC: 3567130. DOI: 10.1371/journal.pone.0055599. View

14.

Kulikov A, Moreau X, Jeanningros R . Effects of experimental hypothyroidism on 5-HT1A, 5-HT2A receptors, 5-HT uptake sites and tryptophan hydroxylase activity in mature rat brain1. Neuroendocrinology. 1999; 69(6):453-9. DOI: 10.1159/000054449. View

15.

Zhang B, Zeng M, Li B, Kan Y, Wang S, Cao B . Arbutin attenuates LPS-induced acute kidney injury by inhibiting inflammation and apoptosis via the PI3K/Akt/Nrf2 pathway. Phytomedicine. 2021; 82:153466. DOI: 10.1016/j.phymed.2021.153466. View

16.

Buriani A, Garcia-Bermejo M, Bosisio E, Xu Q, Li H, Dong X . Omic techniques in systems biology approaches to traditional Chinese medicine research: present and future. J Ethnopharmacol. 2012; 140(3):535-44. DOI: 10.1016/j.jep.2012.01.055. View

17.

Barnes S, Benton H, Casazza K, Cooper S, Cui X, Du X . Training in metabolomics research. I. Designing the experiment, collecting and extracting samples and generating metabolomics data. J Mass Spectrom. 2016; 51(7):461-75. PMC: 4964969. DOI: 10.1002/jms.3782. View

18.

Obregon M . Adipose tissues and thyroid hormones. Front Physiol. 2015; 5:479. PMC: 4263094. DOI: 10.3389/fphys.2014.00479. View

19.

Lu X, Xiong Z, Li J, Zheng S, Huo T, Li F . Metabonomic study on 'Kidney-Yang Deficiency syndrome' and intervention effects of Rhizoma Drynariae extracts in rats using ultra performance liquid chromatography coupled with mass spectrometry. Talanta. 2010; 83(3):700-8. DOI: 10.1016/j.talanta.2010.09.026. View

20.

Sinha R, Singh B, Yen P . Direct effects of thyroid hormones on hepatic lipid metabolism. Nat Rev Endocrinol. 2018; 14(5):259-269. PMC: 6013028. DOI: 10.1038/nrendo.2018.10. View