Composition and Diversity of Gut Microbiota in Diabetic Retinopathy

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Sep 9

PMID 36081798

Authors

Jianhao Bai

Zhongqi Wan

Yuanyuan Zhang

Tianyu Wang

Yawen Xue

Qing Peng

Affiliations

Soon will be listed here.

Abstract

Objective: Diabetic retinopathy (DR) is one of the most common complications of type 2 diabetes mellitus. The current study investigates the composition, structure, and function of gut microbiota in DR patients and explores the correlation between gut microbiota and clinical characteristics of DR.

Methods: A total of 50 stool samples were collected from 50 participants, including 25 DR patients and 25 healthy controls (HCs). 16S ribosomal RNA gene sequencing was used to analyze the gut microbial composition in these two groups. DNA was extracted from the fecal samples using the MiSeq platform.

Results: The microbial structure and composition of DR patients were different from that of HCs. The microbial richness of gut microbiota in DR was higher than that of normal individuals. The alterations of microbiome of DR patients were associated with disrupted Firmicutes, Bacteroidetes, Synergistota, and Desulfobacterota phyla. In addition, increased levels of , , _group, , and , and decreased levels of , _group, , , , , and genera were observed in the DR groups. Additionally, a stochastic forest model was developed to identify a set of biomarkers with seven bacterial genera that can differentiate patients with DR from those HC. The microbial communities exhibited varied functions in these two groups because of the alterations of the above-mentioned bacterial genera.

Conclusion: The altered composition and function of gut microbiota in DR patients indicated that gut microbiome could be used as non-invasive biomarkers, improve clinical diagnostic methods, and identify putative therapeutic targets for DR.

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References

Shivaji S . We are not alone: a case for the human microbiome in extra intestinal diseases. Gut Pathog. 2017; 9:13. PMC: 5339978. DOI: 10.1186/s13099-017-0163-3. View

Turnbaugh P, Ley R, Mahowald M, Magrini V, Mardis E, Gordon J . An obesity-associated gut microbiome with increased capacity for energy harvest. Nature. 2006; 444(7122):1027-31. DOI: 10.1038/nature05414. View

Li Q, Chang Y, Zhang K, Chen H, Tao S, Zhang Z . Implication of the gut microbiome composition of type 2 diabetic patients from northern China. Sci Rep. 2020; 10(1):5450. PMC: 7096501. DOI: 10.1038/s41598-020-62224-3. View

Forslund K, Hildebrand F, Nielsen T, Falony G, Le Chatelier E, Sunagawa S . Disentangling type 2 diabetes and metformin treatment signatures in the human gut microbiota. Nature. 2015; 528(7581):262-266. PMC: 4681099. DOI: 10.1038/nature15766. View

Udayappan S, Manneras-Holm L, Chaplin-Scott A, Belzer C, Herrema H, Dallinga-Thie G . Oral treatment with improves insulin sensitivity in mice. NPJ Biofilms Microbiomes. 2017; 2:16009. PMC: 5515273. DOI: 10.1038/npjbiofilms.2016.9. View

Salamon D, Sroka-Oleksiak A, Kapusta P, Szopa M, Mrozinska S, Ludwig-Slomczynska A . Characteristics of gut microbiota in adult patients with type 1 and type 2 diabetes based on next‑generation sequencing of the 16S rRNA gene fragment. Pol Arch Intern Med. 2018; 128(6):336-343. DOI: 10.20452/pamw.4246. View

Hills Jr R, Pontefract B, Mishcon H, Black C, Sutton S, Theberge C . Gut Microbiome: Profound Implications for Diet and Disease. Nutrients. 2019; 11(7). PMC: 6682904. DOI: 10.3390/nu11071613. View

Hiippala K, Jouhten H, Ronkainen A, Hartikainen A, Kainulainen V, Jalanka J . The Potential of Gut Commensals in Reinforcing Intestinal Barrier Function and Alleviating Inflammation. Nutrients. 2018; 10(8). PMC: 6116138. DOI: 10.3390/nu10080988. View

Larsen N, Vogensen F, van den Berg F, Nielsen D, Andreasen A, Pedersen B . Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PLoS One. 2010; 5(2):e9085. PMC: 2816710. DOI: 10.1371/journal.pone.0009085. View

10.

Wang X, Sun G, Feng T, Zhang J, Huang X, Wang T . Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression. Cell Res. 2019; 29(10):787-803. PMC: 6796854. DOI: 10.1038/s41422-019-0216-x. View

11.

Shang J, Liu F, Zhang B, Dong K, Lu M, Jiang R . Liraglutide-induced structural modulation of the gut microbiota in patients with type 2 diabetes mellitus. PeerJ. 2021; 9:e11128. PMC: 8019531. DOI: 10.7717/peerj.11128. View

12.

Candela M, Biagi E, Soverini M, Consolandi C, Quercia S, Severgnini M . Modulation of gut microbiota dysbioses in type 2 diabetic patients by macrobiotic Ma-Pi 2 diet. Br J Nutr. 2016; 116(1):80-93. PMC: 4894062. DOI: 10.1017/S0007114516001045. View

13.

Zhao L, Lou H, Peng Y, Chen S, Zhang Y, Li X . Comprehensive relationships between gut microbiome and faecal metabolome in individuals with type 2 diabetes and its complications. Endocrine. 2019; 66(3):526-537. DOI: 10.1007/s12020-019-02103-8. View

14.

Caputi V, Giron M . Microbiome-Gut-Brain Axis and Toll-Like Receptors in Parkinson's Disease. Int J Mol Sci. 2018; 19(6). PMC: 6032048. DOI: 10.3390/ijms19061689. View

15.

Moubayed N, Bhat R, Al Farraj D, Dihani N, El Ansary A, Fahmy R . Screening and identification of gut anaerobes (Bacteroidetes) from human diabetic stool samples with and without retinopathy in comparison to control subjects. Microb Pathog. 2019; 129:88-92. DOI: 10.1016/j.micpath.2019.01.025. View

16.

Zheng P, Yang J, Li Y, Wu J, Liang W, Yin B . Gut Microbial Signatures Can Discriminate Unipolar from Bipolar Depression. Adv Sci (Weinh). 2020; 7(7):1902862. PMC: 7140990. DOI: 10.1002/advs.201902862. View

17.

Allin K, Tremaroli V, Caesar R, Jensen B, Damgaard M, Bahl M . Aberrant intestinal microbiota in individuals with prediabetes. Diabetologia. 2018; 61(4):810-820. PMC: 6448993. DOI: 10.1007/s00125-018-4550-1. View

18.

Zhu L, Sha L, Li K, Wang Z, Wang T, Li Y . Dietary flaxseed oil rich in omega-3 suppresses severity of type 2 diabetes mellitus via anti-inflammation and modulating gut microbiota in rats. Lipids Health Dis. 2020; 19(1):20. PMC: 7006389. DOI: 10.1186/s12944-019-1167-4. View

19.

Beli E, Yan Y, Moldovan L, Vieira C, Gao R, Duan Y . Restructuring of the Gut Microbiome by Intermittent Fasting Prevents Retinopathy and Prolongs Survival in Mice. Diabetes. 2018; 67(9):1867-1879. PMC: 6110320. DOI: 10.2337/db18-0158. View

20.

Hao L, Michaelsen T, Singleton C, Dottorini G, Kirkegaard R, Albertsen M . Novel syntrophic bacteria in full-scale anaerobic digesters revealed by genome-centric metatranscriptomics. ISME J. 2020; 14(4):906-918. PMC: 7082340. DOI: 10.1038/s41396-019-0571-0. View