Shall We Tune? From Core-Shell to Cloud Type Nanostructures in Heparin/Silica Hybrids

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2022 Sep 9

PMID 36080642

Authors

Giulio Pota

Giuseppe Vitiello

Virginia Venezia

Francesca Della Sala

Assunta Borzacchiello

Aniello Costantini

Luigi Paduano

Leide P Cavalcanti

Fabiana Tescione

Brigida Silvestri

Giuseppina Luciani

Affiliations

Soon will be listed here.

Abstract

Heparin plays multiple biological roles depending on the availability of active sites strongly influenced by the conformation and the structure of polysaccharide chains. Combining different components at the molecular scale offers an extraordinary chance to easily tune the structural organization of heparin required for exploring new potential applications. In fact, the combination of different material types leads to challenges that cannot be achieved by each single component. In this study, hybrid heparin/silica nanoparticles were synthesized, and the role of silica as a templating agent for heparin supramolecular organization was investigated. The effect of synthesis parameters on particles compositions was deeply investigated by Fourier Transform Infrared Spectroscopy (FTIR) and Thermogravimetric Analysis (TGA). Transmission Electron Microscopy (TEM) reveals a different supramolecular organization of both components, leading to amazing organic-inorganic nanoparticles with different behavior in drug encapsulation and release. Furthermore, favorable biocompatibility for healthy human dermal fibroblasts (HDF) and tumor HS578T cells has been assessed, and a different biological behavior was observed, ascribed to different surface charge and morphology of synthesized nanoparticles.

References

Liu S, Luo H, Li N, Liu Z, Zheng W . Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes. Anal Chem. 2001; 73(16):3907-14. DOI: 10.1021/ac001454h. View

Noti C, Seeberger P . Chemical approaches to define the structure-activity relationship of heparin-like glycosaminoglycans. Chem Biol. 2005; 12(7):731-56. DOI: 10.1016/j.chembiol.2005.05.013. View

Pota G, Venezia V, Vitiello G, Di Donato P, Mollo V, Costantini A . Tuning Functional Behavior of Humic Acids through Interactions with Stöber Silica Nanoparticles. Polymers (Basel). 2020; 12(4). PMC: 7240412. DOI: 10.3390/polym12040982. View

Canta M, Cauda V . The investigation of the parameters affecting the ZnO nanoparticle cytotoxicity behaviour: a tutorial review. Biomater Sci. 2020; 8(22):6157-6174. PMC: 7610635. DOI: 10.1039/d0bm01086c. View

de Almeida M, Susnik E, Drasler B, Taladriz-Blanco P, Petri-Fink A, Rothen-Rutishauser B . Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine. Chem Soc Rev. 2021; 50(9):5397-5434. PMC: 8111542. DOI: 10.1039/d0cs01127d. View

Lin Y, Linask K, Mallon B, Johnson K, Klein M, Beers J . Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem Cells Transl Med. 2017; 6(2):527-538. PMC: 5442822. DOI: 10.5966/sctm.2015-0428. View

Argyo C, Cauda V, Engelke H, Radler J, Bein G, Bein T . Heparin-coated colloidal mesoporous silica nanoparticles efficiently bind to antithrombin as an anticoagulant drug-delivery system. Chemistry. 2011; 18(2):428-32. DOI: 10.1002/chem.201102926. View

Watarai A, Schirmer L, Thones S, Freudenberg U, Werner C, Simon J . TGFβ functionalized starPEG-heparin hydrogels modulate human dermal fibroblast growth and differentiation. Acta Biomater. 2015; 25:65-75. DOI: 10.1016/j.actbio.2015.07.036. View

Simon Ting S, Whitelock J, Tomic R, Gunawan C, Teoh W, Amal R . Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes. Biomaterials. 2013; 34(17):4377-86. DOI: 10.1016/j.biomaterials.2013.02.042. View

10.

Venezia V, Pota G, Silvestri B, Vitiello G, Di Donato P, Landi G . A study on structural evolution of hybrid humic Acids-SiO nanostructures in pure water: Effects on physico-chemical and functional properties. Chemosphere. 2021; 287(Pt 1):131985. DOI: 10.1016/j.chemosphere.2021.131985. View

11.

Bromfield S, Wilde E, Smith D . Heparin sensing and binding - taking supramolecular chemistry towards clinical applications. Chem Soc Rev. 2013; 42(23):9184-95. DOI: 10.1039/c3cs60278h. View

12.

Xiao Z, Levy-Nissenbaum E, Alexis F, Luptak A, Teply B, Chan J . Engineering of targeted nanoparticles for cancer therapy using internalizing aptamers isolated by cell-uptake selection. ACS Nano. 2012; 6(1):696-704. PMC: 3515647. DOI: 10.1021/nn204165v. View

13.

Adnan M, Dalod A, Balci M, Glaum J, Einarsrud M . In Situ Synthesis of Hybrid Inorganic⁻Polymer Nanocomposites. Polymers (Basel). 2019; 10(10). PMC: 6403593. DOI: 10.3390/polym10101129. View

14.

Arlov O, Skjak-Braek G . Sulfated Alginates as Heparin Analogues: A Review of Chemical and Functional Properties. Molecules. 2017; 22(5). PMC: 6154561. DOI: 10.3390/molecules22050778. View

15.

Ma S, Chen Y, Feng J, Liu J, Zuo X, Chen X . One-Step Synthesis of Water-Dispersible and Biocompatible Silicon Nanoparticles for Selective Heparin Sensing and Cell Imaging. Anal Chem. 2016; 88(21):10474-10481. DOI: 10.1021/acs.analchem.6b02448. View

16.

Mei L, Liu Y, Zhang H, Zhang Z, Gao H, He Q . Antitumor and Antimetastasis Activities of Heparin-based Micelle Served As Both Carrier and Drug. ACS Appl Mater Interfaces. 2016; 8(15):9577-89. DOI: 10.1021/acsami.5b12347. View

17.

Della Sala F, Silvestri T, Borzacchiello A, Mayol L, Ambrosio L, Biondi M . Hyaluronan-coated nanoparticles for active tumor targeting: Influence of polysaccharide molecular weight on cell uptake. Colloids Surf B Biointerfaces. 2021; 210:112240. DOI: 10.1016/j.colsurfb.2021.112240. View

18.

Fan X, Domszy R, Hu N, Yang A, Yang J, David A . Synthesis of silica-alginate nanoparticles and their potential application as pH-responsive drug carriers. J Solgel Sci Technol. 2020; 91(1):11-20. PMC: 7451248. DOI: 10.1007/s10971-019-04995-4. View

19.

Silvestri B, Pezzella A, Luciani G, Costantini A, Tescione F, Branda F . Heparin conjugated silica nanoparticle synthesis. Mater Sci Eng C Mater Biol Appl. 2021; 32(7):2037-2041. DOI: 10.1016/j.msec.2012.05.018. View

20.

Vitiello G, Venezia V, Verrillo M, Nuzzo A, Houston J, Cimino S . Hybrid humic acid/titanium dioxide nanomaterials as highly effective antimicrobial agents against gram(-) pathogens and antibiotic contaminants in wastewater. Environ Res. 2020; 193:110562. DOI: 10.1016/j.envres.2020.110562. View