Evaluation of a Chaotrope and Kosmotrope in the Multivariate Optimization of PHW-ATPE of Solasodine from Leaves of

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Sep 9

PMID 36080313

Authors

Tebogo Mphatlalala Mokgehle

Ntakadzeni Edwin Madala

Nikita Tawanda Tavengwa

Affiliations

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Abstract

A hyphenated pressurized hot water—aqueous two-phase extraction (PHW-ATPE) method was applied to extract solasodine from Solanum mauritianum (S. mauritianum). A central composite design (CCD) was applied to determine the optimal conditions for the extraction of solasodine. The parameters evaluated included the percentage concentration of salt (NaCl or Na2CO3) and temperature. The fit of the central composite design response surface model for PHW-ATPE to the data generated a model with a good quadratic fit (R2 = 0.901). The statistically significant (p < 0.05) parameters, such as the linear and quadratic effects of the concentration of salt (%) powder, had a significant impact on the extraction of solasodine. The application of multiply charged salts such as Na2CO3 (kosmotrope) was shown to be a comparably better extractant of solasodine than NaCl (chaotrope) due to the salting-out effect. The optimized conditions for extraction of solasodine with NaCl or Na2CO3 were a temperature of 80 °C at a salt concentration of 20%. The maximum extraction of solasodine was 300.79 mg kg−1 and 162.34 mg kg−1 for Na2CO3 and NaCl, respectively.

References

Hernandez-Mesa M, Cruces-Blanco C, Garcia-Campana A . Simple and rapid determination of 5-nitroimidazoles and metabolites in fish roe samples by salting-out assisted liquid-liquid extraction and UHPLC-MS/MS. Food Chem. 2018; 252:294-302. DOI: 10.1016/j.foodchem.2018.01.101. View

Nuapia Y, Tutu H, Chimuka L, Cukrowska E . Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction. Molecules. 2020; 25(6). PMC: 7144126. DOI: 10.3390/molecules25061335. View

Teo C, Tan S, Yong J, Hew C, Ong E . Pressurized hot water extraction (PHWE). J Chromatogr A. 2010; 1217(16):2484-94. DOI: 10.1016/j.chroma.2009.12.050. View

Silva C, Perestrelo R, Silva P, Tomas H, Camara J . Implementing a central composite design for the optimization of solid phase microextraction to establish the urinary volatomic expression: a first approach for breast cancer. Metabolomics. 2019; 15(4):64. DOI: 10.1007/s11306-019-1525-2. View

Luo Q, Zhang J, Li H, Wu D, Geng F, Corke H . Green Extraction of Antioxidant Polyphenols from Green Tea (). Antioxidants (Basel). 2020; 9(9). PMC: 7555212. DOI: 10.3390/antiox9090785. View

Gbashi S, Njobeh P, Steenkamp P, Tutu H, Madala N . The effect of temperature and methanol-water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa. Chem Cent J. 2018; 10(1):37. PMC: 5396525. DOI: 10.1186/s13065-016-0182-z. View

Sazali N, Alshishani A, Saad B, Chew K, Chong M, Miskam M . Salting-out assisted liquid-liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples. R Soc Open Sci. 2019; 6(8):190952. PMC: 6731698. DOI: 10.1098/rsos.190952. View

Gbashi S, Njobeh P, De Saeger S, De Boevre M, Madala N . Development, chemometric-assisted optimization and in-house validation of a modified pressurized hot water extraction methodology for multi-mycotoxins in maize. Food Chem. 2019; 307:125526. DOI: 10.1016/j.foodchem.2019.125526. View

Musarurwa H, Tavengwa N . Emerging green solvents and their applications during pesticide analysis in food and environmental samples. Talanta. 2020; 223(Pt 1):121507. DOI: 10.1016/j.talanta.2020.121507. View

10.

Rodrigues T, Morais D, Gianvecchio V, Aquino E, Cunha R, Huestis M . Development and Validation of a Method for Quantification of 28 Psychotropic Drugs in Postmortem Blood Samples by Modified Micro-QuEChERS and LC-MS-MS. J Anal Toxicol. 2020; 45(7):644-656. DOI: 10.1093/jat/bkaa138. View

11.

Fekry M, Ezzat S, Salama M, Alshehri O, Al-Abd A . Bioactive glycoalkaloides isolated from Solanum melongena fruit peels with potential anticancer properties against hepatocellular carcinoma cells. Sci Rep. 2019; 9(1):1746. PMC: 6370831. DOI: 10.1038/s41598-018-36089-6. View

12.

Neves C, Sousa R, Pereira M, Freire M, Coutinho J . Understanding the Effect of Ionic Liquids as Adjuvants in the Partition of Biomolecules in Aqueous Two-Phase Systems Formed by Polymers and Weak Salting-Out Agents. Biochem Eng J. 2019; 141:239-246. PMC: 6443036. DOI: 10.1016/j.bej.2018.10.022. View

13.

Tejada-Casado C, Olmo-Iruela M, Garcia-Campana A, Lara F . Green and simple analytical method to determine benzimidazoles in milk samples by using salting-out assisted liquid-liquid extraction and capillary liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2018; 1091:46-52. DOI: 10.1016/j.jchromb.2018.05.024. View

14.

Burato J, Medina D, de Toffoli A, Maciel E, Lancas F . Recent advances and trends in miniaturized sample preparation techniques. J Sep Sci. 2019; 43(1):202-225. DOI: 10.1002/jssc.201900776. View

15.

Arteaga-Crespo Y, Radice M, Bravo-Sanchez L, Garcia-Quintana Y, Scalvenzi L . Optimisation of ultrasound-assisted extraction of phenolic antioxidants from Loes. leaves using response surface methodology. Heliyon. 2020; 6(1):e03043. PMC: 6939111. DOI: 10.1016/j.heliyon.2019.e03043. View

16.

Lendor S, Hassani S, Boyaci E, Singh V, Womelsdorf T, Pawliszyn J . Solid Phase Microextraction-Based Miniaturized Probe and Protocol for Extraction of Neurotransmitters from Brains in Vivo. Anal Chem. 2019; 91(7):4896-4905. DOI: 10.1021/acs.analchem.9b00995. View