The Fruit Glossiness Locus, (), Encodes a CH-type Zinc Finger Transcription Factor, CsDULL, in Cucumber ( L.)

Overview

Journal Hortic Res

Publisher Oxford University Press

Date 2022 Sep 8

PMID 36072836

Authors

Xuling Zhai

Haoying Wu

Yaru Wang

Zhongren Zhang

Li Shan

Xi Zhao

Ruijia Wang

Chang Liu

Yiqun Weng

Ying Wang

Xingwang Liu

Huazhong Ren

Affiliations

Soon will be listed here.

Abstract

Fruit glossiness is an important external fruit quality trait for fresh-consumed cucumber fruit, affecting its marketability. Dull fruit appearance is mainly controlled by a single gene, (for ) that is dominant to glossy fruit (), but the molecular mechanism controlling fruit glossiness is unknown. In the present study, we conducted map-based cloning of the locus in cucumber and identified a candidate gene () that encodes a CH-type zinc finger transcription factor, CsDULL. A 4895-bp deletion including the complete loss of resulted in glossy fruit. is highly expressed in the peel of cucumber fruit, and its expression level is positively correlated with the accumulation of cutin and wax in the peel. Through transcriptome analysis, yeast one-hybrid and dual-luciferase assays, we identified two genes potentially targeted by CsDULL for regulation of cutin and wax biosynthesis/transportation that included and . The possibility that CsDULL controls both fruit glossiness and wart development in cucumber is discussed. The present work advances our understanding of regulatory mechanisms of fruit epidermal traits, and provides a useful tool for molecular breeding to improve external fruit quality in cucumber.

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