Mapping QTLs for Yield and Photosynthesis-related Traits in Three Consecutive Backcross Populations of Oryza Sativa Cultivar Cottondora Sannalu (MTU1010) and Oryza Rufipogon

Overview

Journal Planta

Specialty Biology

Date 2022 Sep 7

PMID 36070104

Authors

Venkateswara Rao Yadavalli

Divya Balakrishnan

Malathi Surapaneni

Krishnamraju Addanki

Sukumar Mesapogu

Kavitha Beerelli

Subrahmanyam Desiraju

Sitapati Rao Voleti

Sarla Neelamraju

Affiliations

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Abstract

Identification of trait enhancing QTLs for yield and photosynthesis-related traits in rice using interspecific mapping population and chromosome segment substitution lines derived from a cross between Oryza sativa and Oryza rufipogon. Wild rice contains novel genes which can help in improving rice yield. Common wild rice Oryza rufipogon is a known source for enhanced photosynthesis and yield-related traits. We developed BCF mapping populations using O. rufipogon IC309814 with high photosynthetic rate as donor, and elite cultivar MTU1010 as recurrent parent. Evaluation of 238 BCF families for 13 yield-related traits and 208 BCF families for seven photosynthesis-related physiological traits resulted in identification of significantly different lines which performed better than MTU1010 for various yield contributing traits. 49 QTLs were identified for 13 yield traits and 7 QTLs for photosynthesis-related traits in BCF In addition, 34 QTLs in BCF and 26 QTLs in BCF were also detected for yield traits.11 common QTLs were identified in three consecutive generations and their trait-increasing alleles were derived from O. rufipogon. Significantly, one major effect common QTL qTGW3.1 for thousand grain weight with average phenotypic variance 8.1% and one novel QTL qBM7.1 for biomass were identified. Photosynthesis-related QTLs qP9.1, qP12.1, qP12.2 qSPAD1.1 and qSPAD6.1 showed additive effect from O. rufipogon. A set of 145 CSSLs were identified in BCF which together represented 87% of O. rufipogon genome. In addition, 87 of the 145 CSSLs were significantly different than MTU1010 for at least one trait. The major effect QTLs can be fine mapped for gene discovery. CSSLs developed in this study are a good source of novel alleles from O. rufipogon in the background of Cottondora Sannalu for rapid improvement of any trait in rice.

Citing Articles

Fine mapping of interspecific secondary CSSL populations revealed key regulators for grain weight at locus from .

Surapaneni M, Balakrishnan D, Addanki K, Yadavalli V, Kumar A, Prashanthi P Physiol Mol Biol Plants. 2024; 30(7):1145-1160.

PMID: 39100880 PMC: 11291809. DOI: 10.1007/s12298-024-01483-0.

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