Metagenomic Next-generation Sequencing in Diagnosing Pneumonia: A Case Report

Overview

Journal Open Life Sci

Specialty Biology

Date 2022 Sep 5

PMID 36060643

Authors

Yuan Zhang

Zhaoshang Zeng

FengHui Li

Zhiyun Peng

Han Xia

Yunyi Zeng

Haimin Chen

Yingjing Wang

Weining Xie

Yanhua Zhang

Zhongxiang Tang

Affiliations

Soon will be listed here.

Abstract

It remains a huge challenge for clinicians to diagnose pneumonia (PJP) by a conventional method, which leads to delay in diagnosing PJP, accounting for higher mortality in patients with rheumatoid arthritis (RA). A 69-year-old woman, who suffered from RA for years, developed acute respiratory failure. The computed tomography scan showed diffused effusion and ground glass opacity in both lungs, which could not be differentiated from interstitial pneumonia. Metagenomic next-generation sequencing (mNGS) revealed in both serum and bronchoalveolar lavage fluid with reads per million (RPM) of 17 and 437, while other diagnostic tests did not detect any pathogenic microorganism. The results were verified by quantitative polymerase chain reaction (mtSSU region) against the same samples. The DNA RPM of declined notably after treatment with trimethoprim/sulfamethoxazole. The patient was discharged without treatment and finally passed away. This case fully highlights the sensitivity of mNGS in early diagnosis of PJP, which is of great significance for prognosis and treatment. Nonetheless, the clinical application of mNGS is worth further standardization and normalization.

Citing Articles

A Case Report of Cutaneous Anthrax Diagnosed by Using a Metagenomic Next-Generation Sequencing (mNGS) Approach.

Liu Y, Zheng G, Li J, Yang N, Li J, Liu Z Infect Drug Resist. 2023; 16:3601-3606.

PMID: 37309378 PMC: 10257910. DOI: 10.2147/IDR.S411717.

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