Regulatory T Cells Regulate Blastemal Proliferation During Zebrafish Caudal Fin Regeneration

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2022 Sep 5

PMID 36059461

Authors

Subhra P Hui

Kotaro Sugimoto

Delicia Z Sheng

Kazu Kikuchi

Affiliations

Soon will be listed here.

Abstract

The role of T cells in appendage regeneration remains unclear. In this study, we revealed an important role for regulatory T cells (Tregs), a subset of T cells that regulate tolerance and tissue repair, in the epimorphic regeneration of zebrafish caudal fin tissue. Upon amputation, fin tissue-resident Tregs infiltrate into the blastema, a population of progenitor cells that produce new fin tissues. Conditional genetic ablation of Tregs attenuates blastemal cell proliferation during fin regeneration. Blastema-infiltrating Tregs upregulate the expression of and , and pharmacological activation of IGF signaling restores blastemal proliferation in Treg-ablated zebrafish. These findings further extend our understandings of Treg function in tissue regeneration and repair.

Citing Articles

Adipose-tissue Treg cells restrain differentiation of stromal adipocyte precursors to promote insulin sensitivity and metabolic homeostasis.

Wang G, Munoz-Rojas A, Spallanzani R, Franklin R, Benoist C, Mathis D Immunity. 2024; 57(6):1345-1359.e5.

PMID: 38692280 PMC: 11188921. DOI: 10.1016/j.immuni.2024.04.002.

References

Ang N, Saera-Vila A, Walsh C, Hitchcock P, Kahana A, Thummel R . Midkine-a functions as a universal regulator of proliferation during epimorphic regeneration in adult zebrafish. PLoS One. 2020; 15(6):e0232308. PMC: 7292404. DOI: 10.1371/journal.pone.0232308. View

Kragl M, Knapp D, Nacu E, Khattak S, Maden M, Epperlein H . Cells keep a memory of their tissue origin during axolotl limb regeneration. Nature. 2009; 460(7251):60-5. DOI: 10.1038/nature08152. View

Knopf F, Hammond C, Chekuru A, Kurth T, Hans S, Weber C . Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin. Dev Cell. 2011; 20(5):713-24. DOI: 10.1016/j.devcel.2011.04.014. View

Nechiporuk A, Keating M . A proliferation gradient between proximal and msxb-expressing distal blastema directs zebrafish fin regeneration. Development. 2002; 129(11):2607-17. DOI: 10.1242/dev.129.11.2607. View

Iribarne M . Inflammation induces zebrafish regeneration. Neural Regen Res. 2021; 16(9):1693-1701. PMC: 8328752. DOI: 10.4103/1673-5374.306059. View

Bodnar R . Epidermal Growth Factor and Epidermal Growth Factor Receptor: The Yin and Yang in the Treatment of Cutaneous Wounds and Cancer. Adv Wound Care (New Rochelle). 2014; 2(1):24-29. PMC: 3840479. DOI: 10.1089/wound.2011.0326. View

Poss K, Keating M, Nechiporuk A . Tales of regeneration in zebrafish. Dev Dyn. 2003; 226(2):202-10. DOI: 10.1002/dvdy.10220. View

Gemberling M, Bailey T, Hyde D, Poss K . The zebrafish as a model for complex tissue regeneration. Trends Genet. 2013; 29(11):611-20. PMC: 3812420. DOI: 10.1016/j.tig.2013.07.003. View

Chablais F, Jazwinska A . IGF signaling between blastema and wound epidermis is required for fin regeneration. Development. 2010; 137(6):871-9. DOI: 10.1242/dev.043885. View

10.

Hasegawa T, Hall C, Crosier P, Abe G, Kawakami K, Kudo A . Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. Elife. 2017; 6. PMC: 5360449. DOI: 10.7554/eLife.22716. View

11.

Trede N, Langenau D, Traver D, Look A, Zon L . The use of zebrafish to understand immunity. Immunity. 2004; 20(4):367-79. DOI: 10.1016/s1074-7613(04)00084-6. View

12.

Shi L, Chen C, Yin Z, Wei G, Xie G, Liu D . Systematic profiling of early regulators during tissue regeneration using zebrafish model. Wound Repair Regen. 2020; 29(1):189-195. DOI: 10.1111/wrr.12852. View

13.

Tal T, Franzosa J, Tanguay R . Molecular signaling networks that choreograph epimorphic fin regeneration in zebrafish - a mini-review. Gerontology. 2009; 56(2):231-40. PMC: 2969159. DOI: 10.1159/000259327. View

14.

Curado S, Anderson R, Jungblut B, Mumm J, Schroeter E, Stainier D . Conditional targeted cell ablation in zebrafish: a new tool for regeneration studies. Dev Dyn. 2007; 236(4):1025-35. DOI: 10.1002/dvdy.21100. View

15.

Kintner C, Brockes J . Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration. Nature. 1984; 308(5954):67-9. DOI: 10.1038/308067a0. View

16.

Chen A, Han Y, Poss K . Regulation of zebrafish fin regeneration by vitamin D signaling. Dev Dyn. 2020; 250(9):1330-1339. PMC: 8050121. DOI: 10.1002/dvdy.261. View

17.

Akimenko M, Mari-Beffa M, Becerra J, Geraudie J . Old questions, new tools, and some answers to the mystery of fin regeneration. Dev Dyn. 2003; 226(2):190-201. DOI: 10.1002/dvdy.10248. View

18.

Petrie T, Strand N, Yang C, Tsung-Yang C, Rabinowitz J, Moon R . Macrophages modulate adult zebrafish tail fin regeneration. Development. 2014; 141(13):2581-91. PMC: 4067955. DOI: 10.1242/dev.098459. View

19.

Li L, Yan B, Shi Y, Zhang W, Wen Z . Live imaging reveals differing roles of macrophages and neutrophils during zebrafish tail fin regeneration. J Biol Chem. 2012; 287(30):25353-60. PMC: 3408142. DOI: 10.1074/jbc.M112.349126. View

20.

Kawakami A . Stem cell system in tissue regeneration in fish. Dev Growth Differ. 2009; 52(1):77-87. DOI: 10.1111/j.1440-169X.2009.01138.x. View