Decoding the Olfactory Map Through Targeted Transcriptomics Links Murine Olfactory Receptors to Glomeruli

Overview

Journal Nat Commun

Specialty Biology

Date 2022 Sep 1

PMID 36050313

Authors

Kevin W Zhu

Shawn D Burton

Maira H Nagai

Justin D Silverman

Claire A de March

Matt Wachowiak

Hiroaki Matsunami

Affiliations

Soon will be listed here.

Abstract

Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position.

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