Taohong Siwu Decoction Attenuates AIM2 and NLRC4 Inflammasomes by Ameliorates Deoxyribonucleic Acid Damage After Ischemic Stroke

Overview

Journal Front Pharmacol

Date 2022 Aug 29

PMID 36034843

Authors

Ni Wang

Furui Chu

Lijuan Zhang

Changyi Fei

Chao Yu

Sujun Xue

Yongzhong Wang

Ling Fang

Daiyin Peng

Xianchun Duan

Weidong Chen

Affiliations

Soon will be listed here.

Abstract

Taohong siwu decoction (THSWD) has been shown to have a therapeutic effect on ischemic strokes (IS). However, it is not clear to us whether THSWD reduces deoxyribonucleic acid (DNA) damage after stroke and reduces the inflammatory response caused by the damage. Therefore, we constructed an IS model (I/R) in rats and performed oxygen-glucose deprivation/reoxygenation (OGD/R) on BV2 cells. Then ELISA, immunofluorescence staining, immunohistochemistry staining, and RT-qPCR were performed to detect the expressions of absent in melanoma 2 (AIM2), NLRC4, and Caspase-1 inflammasomes and other inflammatory factors. Experimental stroke causes DNA damage, and we found that the aforementioned inflammasomes as well as inflammatory factors were significantly inhibited after treatment with THSWD by comparing the model group with the model administration group. In addition, we examined the expression of AIM2, NLRC4, and Caspase-1 in BV2 cells of OGD/R and found that the expression of the aforementioned inflammasomes was significantly decreased in OGD/R by administration of THSWD-containing serum. Our data suggest that THSWD can reduced DNA damage after stroke as well as the inflammatory response caused by the damage.

Citing Articles

Protective effect of Tao Hong Si Wu Decoction against inflammatory injury caused by intestinal flora disorders in an ischemic stroke mouse model.

Zhang L, Xue S, Fei C, Yu C, Li J, Li Y BMC Complement Med Ther. 2024; 24(1):124.

PMID: 38500092 PMC: 10946105. DOI: 10.1186/s12906-024-04417-1.

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