Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat

Overview

Journal Life (Basel)

Specialty Biology

Date 2022 Aug 26

PMID 36013400

Authors

Ahmed Sultan

Ernest Adeghate

Bright Starling Emerald

Muhammad A Qureshi

Saeed Tariq Minhas

Frank Christopher Howarth

Affiliations

Soon will be listed here.

Abstract

(1) Background: Cardiovascular complications are a leading cause of morbidity and mortality in diabetic patients. The effects of obesity and diabesity on the function and structure of ventricular myocytes in the Zucker fatty (ZF) rat and the Zucker diabetic fatty (ZDF) rat compared to Zucker lean (ZL) control rats have been investigated. (2) Methods: Shortening and intracellular Ca were simultaneously measured with cell imaging and fluorescence photometry, respectively. Ventricular muscle protein expression and structure were investigated with Western blot and electron microscopy, respectively. (3) Results: The amplitude of shortening was increased in ZF compared to ZL but not compared to ZDF myocytes. Resting Ca was increased in ZDF compared to ZL myocytes. Time to half decay of the Ca transient was prolonged in ZDF compared to ZL and was reduced in ZF compared to ZL myocytes. Changes in expression of proteins associated with cardiac muscle contraction are presented. Structurally, there were reductions in sarcomere length in ZDF and ZF compared to ZL and reductions in mitochondria count in ZF compared to ZDF and ZL myocytes. (4) Conclusions: Alterations in ventricular muscle proteins and structure may partly underlie the defects observed in Ca signaling in ZDF and ZF compared to ZL rat hearts.

Citing Articles

Regulation of Prepro-NeuropeptideW/B and Its Receptor in the Heart of ZDF Rats: An Animal Model of Type II DM.

Pandey S, Jarkovska D, Tuma Z, Smrhova T, Chottova Dvorakova M Int J Mol Sci. 2022; 23(23).

PMID: 36499546 PMC: 9739957. DOI: 10.3390/ijms232315219.

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