Epiphytic Yeasts and Bacteria As Candidate Biocontrol Agents of Green and Blue Molds of Citrus Fruits

Overview

Journal J Fungi (Basel)

Date 2022 Aug 26

PMID 36012806

Authors

Rania Hammami

Maroua Oueslati

Marwa Smiri

Souhaila Nefzi

Mustapha Ruissi

Francesca Comitini

Gianfranco Romanazzi

Santa Olga Cacciola

Najla Sadfi Zouaoui

Affiliations

Soon will be listed here.

Abstract

Overall, 180 yeasts and bacteria isolated from the peel of citrus fruits were screened for their in vitro antagonistic activity against and , causative agents of green and blue mold of citrus fruits, respectively. Two yeast and three bacterial isolates were selected for their inhibitory activity on mycelium growth. Based on the phylogenetic analysis of 16S rDNA and ITS rDNA sequences, the yeast isolates were identified as and while the bacterial isolates were identified as , and . All five selected isolates significantly reduced the incidence of decay incited by and on 'Valencia' orange and 'Eureka' lemon fruits. Moreover, they were effective in preventing natural infections of green and blue mold of fruits stored at 4 °C. Treatments with antagonistic yeasts and bacteria did not negatively affect the quality and shelf life of fruits. The antagonistic efficacy of the five isolates depended on multiple modes of action, including the ability to form biofilms and produce antifungal lipopeptides, lytic enzymes and volatile compounds. The selected isolates are promising as biocontrol agents of postharvest green and blue molds of citrus fruits.

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