Frequency of β-Casein Gene Polymorphisms in Jersey Cows in Western Japan

Overview

Journal Animals (Basel)

Date 2022 Aug 26

PMID 36009666

Authors

Nuomin

Qui Dang Nguyen

AoDaohu

Naoki Nishino

Affiliations

Soon will be listed here.

Abstract

This study aimed to investigate β-casein gene polymorphisms in Jersey cows in Japan. Blood samples were collected from 590 cows from eight Jersey farms in Okayama Prefecture, western Japan. Sequence analysis of exon 7 regions in chromosome 6 of the gene revealed the genotype and allele frequencies of the β-casein variants. Considering that variant B belongs to the A1 group and variant I to the A2 group, plasma metabolite concentrations were compared among the A1A1, A1A2, and A2A2 group-based genotypes. The most frequent genotype was A2A2 (0.558), followed by A2B (0.190) and A2I (0.103). No variants of A3, F, G, H1, or H2 were found. The frequencies of group-based genotypes were A1A1 (0.032), A1A2 (0.303), and A2A2 (0.665). Although farm-to-farm differences were observed in the plasma concentrations of urea nitrogen, calcium, and phosphorus, no differences were found between the A1A1, A1A2, and A2A2 group-based genotypes; hence, the β-casein genotypes did not affect the metabolism of major nutrients. Owing to the high frequency of the A2 variant, Jersey cows can be considered an attractive breed for marker-assisted selection to create A2A2 herds.

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References

Mohan G, Kumar A, Khan S, Kumar N, Kapila S, Lathwal S . Casein (CSN) gene variants and parity affect the milk protein traits in crossbred (Bos taurus x Bos indicus) cows in sub-tropical climate. Trop Anim Health Prod. 2021; 53(2):289. DOI: 10.1007/s11250-021-02736-w. View

Massella E, Piva S, Giacometti F, Liuzzo G, Zambrini A, Serraino A . Evaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy. Ital J Food Saf. 2017; 6(3):6904. PMC: 5641661. DOI: 10.4081/ijfs.2017.6904. View

Yamada A, Sugimura M, Kuramoto T . Genetic polymorphism of bovine beta-casein gene in Japanese dairy farm herds. Anim Sci J. 2021; 92(1):e13644. PMC: 9286554. DOI: 10.1111/asj.13644. View

Dai R, Fang Y, Zhao W, Liu S, Ding J, Xu K . Identification of alleles and genotypes of beta-casein with DNA sequencing analysis in Chinese Holstein cow. J Dairy Res. 2016; 83(3):312-6. DOI: 10.1017/S0022029916000303. View

Elferink A, Entiriwaa D, Bulgarelli P, Smits N, Peters J . Development of a Microsphere-Based Immunoassay Authenticating A2 Milk and Species Purity in the Milk Production Chain. Molecules. 2022; 27(10). PMC: 9144198. DOI: 10.3390/molecules27103199. View

Cieslinska A, Fiedorowicz E, Zwierzchowski G, Kordulewska N, Jarmolowska B, Kostyra E . Genetic Polymorphism of β-Casein Gene in Polish Red Cattle-Preliminary Study of A1 and A2 Frequency in Genetic Conservation Herd. Animals (Basel). 2019; 9(6). PMC: 6616616. DOI: 10.3390/ani9060377. View

Rangel A, Zaros L, Lima T, Borba L, Novaes L, Mota L . Polymorphism in the Beta Casein Gene and analysis of milk characteristicsin Gir and Guzerá dairy cattle. Genet Mol Res. 2017; 16(2). DOI: 10.4238/gmr16029592. View

Kullenberg de Gaudry D, Lohner S, Schmucker C, Kapp P, Motschall E, Horrlein S . Milk A1 β-casein and health-related outcomes in humans: a systematic review. Nutr Rev. 2019; 77(5):278-306. DOI: 10.1093/nutrit/nuy063. View

Kaminski S, Cieslinska A, Kostyra E . Polymorphism of bovine beta-casein and its potential effect on human health. J Appl Genet. 2007; 48(3):189-98. DOI: 10.1007/BF03195213. View

10.

Antonopoulos D, Vougiouklaki D, Laliotis G, Tsironi T, Valasi I, Chatzilazarou A . Identification of Polymorphisms of the Gene Encoding β-Casein in Greek Local Breeds of Cattle. Vet Sci. 2021; 8(11). PMC: 8625921. DOI: 10.3390/vetsci8110257. View

11.

Sebastiani C, Arcangeli C, Ciullo M, Torricelli M, Cinti G, Fisichella S . Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals (Basel). 2020; 10(2). PMC: 7070732. DOI: 10.3390/ani10020252. View

12.

Visker M, Dibbits B, Kinders S, van Valenberg H, van Arendonk J, Bovenhuis H . Association of bovine β-casein protein variant I with milk production and milk protein composition. Anim Genet. 2014; 42(2):212-8. DOI: 10.1111/j.1365-2052.2010.02106.x. View

13.

Kuellenberg de Gaudry D, Lohner S, Bischoff K, Schmucker C, Hoerrlein S, Roeger C . A1- and A2 beta-casein on health-related outcomes: a scoping review of animal studies. Eur J Nutr. 2021; 61(1):1-21. PMC: 8783860. DOI: 10.1007/s00394-021-02551-x. View

14.

Gustavsson F, Buitenhuis A, Johansson M, Bertelsen H, Glantz M, Poulsen N . Effects of breed and casein genetic variants on protein profile in milk from Swedish Red, Danish Holstein, and Danish Jersey cows. J Dairy Sci. 2014; 97(6):3866-77. DOI: 10.3168/jds.2013-7312. View

15.

Caroli A, Savino S, Bulgari O, Monti E . Detecting β-Casein Variation in Bovine Milk. Molecules. 2016; 21(2):141. PMC: 6273733. DOI: 10.3390/molecules21020141. View

16.

Molee A, Boonek L, Rungsakinnin N . The effect of beta and kappa casein genes on milk yield and milk composition in different percentages of Holstein in crossbred dairy cattle. Anim Sci J. 2011; 82(4):512-6. DOI: 10.1111/j.1740-0929.2011.00879.x. View