Halo- and Thiocarbazomycins from Coral- and Coral Reef Sands-Derived

Four actinomycete strains isolated from the coral and coral sand samples from the South China Sea, were found to produce a series of halogenated compounds baring similar ultraviolet absorption based on the analysis of HPLC and LC-MS. The production titers of halogenated compounds from SCSIO 64983 exceeded those of other similar strains leading us to focus on SCSIO 64983. Four new thiocarbazomycins A-B (-), chlocarbazomycin E (), and brocarbazomycin A (), together with three known chlocarbazomycins A-C (-) containing a carbazole core were identified, and their structures were determined using a combination of spectroscopic analysis including HRESIMS, 1D and 2D NMR. Structurally speaking, compounds and have the rare sulfur-containing carbazole nuclei, and and contain Cl and Br atoms, respectively. Although these compounds have not yet been found to have obvious biological activity, their discovery highlights the role of molecular libraries in subsequent drug discovery campaigns.