Differences in Supragingival Microbiome in Patients with and Without Full-Crown Prostheses

Overview

Journal Dent J (Basel)

Specialty Dentistry

Date 2022 Aug 25

PMID 36005250

Authors

Manli Guo

Zhidong Zhang

Jiyuan Lu

Di Wang

Yimin Yan

Shen Zhang

Xin Yu

Songhua Su

Lu Yuan

Zhige Li

Baoping Zhang

Affiliations

Soon will be listed here.

Abstract

To characterize the microflora profile of supragingival biofilm in patients with and without full-crown prostheses. Plaque samples of full-crown prostheses and teeth in patients with porcelain-fused-to-metal crowns, all-ceramic crowns, and no prostheses were collected (three patients per group), using 16S rRNA high-throughput sequencing technology to conduct DNA sequencing on the samples and using Qiime, R, and PICRUSt2 software to perform bioinformatics analyses and functional analyses on sequencing data. In total, 110,209 valid sequences were obtained in the experiment, corresponding to 11 phyla and 120 genera. The predominant species shared by the three groups were phyla , , , , and and genera , , , , , , , , and . The species-difference analysis showed that genus significantly increased after the patient wore the dental prosthesis. Compared with the no-prosthesis samples, the functional analysis showed that cell motility increased in the samples from full-crown prostheses, while replication and repair, and translation decreased. This study reveals the changes in the oral microbial community of patients with full-crown prostheses, which could provide insights regarding the safety of materials for long-term use in the oral cavity.

Citing Articles

Isolation of Clinical Microbial Isolates during Orthodontic Aligner Therapy and Their Ability to Form Biofilm.

Baybekov O, Stanishevskiy Y, Sachivkina N, Bobunova A, Zhabo N, Avdonina M Dent J (Basel). 2023; 11(1).

PMID: 36661550 PMC: 9858208. DOI: 10.3390/dj11010013.

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