A Gene Circuit Combining the Endogenous I-E Type CRISPR-Cas System and a Light Sensor to Produce Poly-β-Hydroxybutyric Acid Efficiently

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2022 Aug 25

PMID 36005038

Authors

Xiaomeng Li

Wei Jiang

Qingsheng Qi

Quanfeng Liang

Affiliations

Soon will be listed here.

Abstract

'Metabolic burden,' which arises when introducing exogenic synthesizing pathways into a host strain, remains a challenging issue in metabolic engineering. Redirecting metabolic flux from cell growth to product synthesis at an appropriate culture timepoint is ideal for resolving this issue. In this report, we introduce optogenetics-which is capable of precise temporal and spatial control-as a genetic switch, accompanied by the endogenous type I-E CRISPRi system in () to generate a metabolic platform that redirects metabolic flux. Poly-β-hydroxybutyric acid (PHB) production was taken as an example to demonstrate the performance of this platform. A two-to-three-fold increase in PHB content was observed under green light when compared with the production of PHB under red light, confirming the regulatory activity of this platform and its potential to redirect metabolic flux to synthesize target products.

Citing Articles

Application of CRISPR Cas Systems for Biosensing.

Liu C, Dai Y Biosensors (Basel). 2023; 13(7).

PMID: 37504071 PMC: 10376997. DOI: 10.3390/bios13070672.

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