Selection of Potential Reference Genes for RT-qPCR in the Plant Pathogenic Fungus

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Aug 25

PMID 36003927

Authors

Xingzhou Chen

Xinggang Chen

Qian Tan

Yuan He

Zhikai Wang

Guoying Zhou

Junang Liu

Affiliations

Soon will be listed here.

Abstract

is widespread, and these pathogenic fungi can cause various plant diseases. Studies have shown that cause oil-tea () anthracnose and is widely distributed as a dominant fungus in all -producing regions. Real-time quantitative PCR(RT-qPCR) is considered the most reliable technique for simultaneously measuring relative gene expression levels in different tissues. Target genes are typically quantified using RT-qPCR to explore gene function, and reliable RT-qPCR results require data normalization using stable reference genes. No studies have reported a suitable reference gene in . This study has eight candidate reference genes (, , , , , , and ) which were selected from - transcriptome data and evaluated and sequenced using geNorm, NormFinder, and BestKeeper algorithms. The results showed that had better stability in , both during the growth of pure pathogenic fungi and during the invasion of different oil-tea leaves. After normalization with , the differentially expressed target genes were similar to the transcriptome. Our work provides suitable reference genes for future studies to quantify target gene expression levels in

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