Label-free Chemical Imaging of Cytochrome P450 Activity by Raman Microscopy

Overview

Journal Commun Biol

Specialty Biology

Date 2022 Aug 22

PMID 35995965

Authors

Menglu Li

Yasunori Nawa

Seiichi Ishida

Yasunari Kanda

Satoshi Fujita

Katsumasa Fujita

Affiliations

Soon will be listed here.

Abstract

Although investigating drug modulation of cytochrome P450 (CYP) activity under physiological conditions is crucial in drug development to avoid severe adverse drug reactions, the current evaluation approaches that rely on the destructive and end-point analysis can be misleading due to invasive treatments and cellular heterogeneity. Here, we propose a non-destructive and high-content method for visualizing and quantifying intracellular CYP activity under drug administration by Raman microscopy. The redox-state and spin-state sensitive Raman measurement indicated that the induced CYPs in living hepatocytes were in oxidized and low-spin state, which is related to monooxygenase function of CYP. Moreover, glycogen depletion associated with CYP induction was simultaneously observed, indicating a relevant effect on glucose metabolism. By deciphering the overall changes in the biochemical fingerprints of hepatocytes, Raman microscopy offers a non-destructive and quantitative chemical imaging method to evaluate CYP activity at the single-cell level with the potential to facilitate future drug development schemes.

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