Transcriptomic Heterogeneity of Cultured ADSCs Corresponds to Embolic Risk in the Host

Overview

Journal iScience

Publisher Cell Press

Date 2022 Aug 22

PMID 35992088

Authors

Kaijing Yan

Jinlai Zhang

Wen Yin

Jeffrey N Harding

Fei Ma

Di Wu

Haibo Deng

Pengfei Han

Rui Li

Hongxu Peng

Xin Song

Y James Kang

Affiliations

Soon will be listed here.

Abstract

Stem cell therapy emerges as an effective approach for treating various currently untreatable diseases. However, fatal and unknown risks caused by their systemic use remain to be a major obstacle to clinical application. We developed a functional single-cell RNA sequencing (scRNA-seq) procedure and identified that transcriptomic heterogeneity of adipose-derived stromal cells (ADSCs) in cultures is responsible for a fatal embolic risk of these cells in the host. The pro-embolic subpopulation of ADSCs in cultures was sorted by gene set enrichment analysis (GSEA) and verified by a supervised machine learning analysis. A mathematical model was developed and validated for the prediction of embolic risk of cultured ADSCs in animal models and further confirmed by its application to public data. Importantly, modification of culture conditions prevented the embolic risk. This novel procedure can be applied to other aspects of risk assessment and would help further the development of stem cell clinical applications.

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