Autocrine/Paracrine Slit-Robo Signaling Controls Optic Lobe Development in

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2022 Aug 19

PMID 35982851

Authors

M Constanza Gonzalez-Ramirez

Francisca Rojo-Cortes

Noemi Candia

Jorge Garay-Montecinos

Pablo Guzman-Palma

Jorge M Campusano

Carlos Oliva

Affiliations

Soon will be listed here.

Abstract

Cell segregation mechanisms play essential roles during the development of the central nervous system (CNS) to support its organization into distinct compartments. The Slit protein is a secreted signal, classically considered a paracrine repellent for axonal growth through Robo receptors. However, its function in the compartmentalization of CNS is less explored. In this work, we show that Slit and Robo3 are expressed in the same neuronal population of the optic lobe, where they are required for the correct compartmentalization of optic lobe neuropils by the action of an autocrine/paracrine mechanism. We characterize the endocytic route followed by the Slit/Robo3 complex and detected genetic interactions with genes involved in endocytosis and actin dynamics. Thus, we report that the Slit-Robo3 pathway regulates the morphogenesis of the optic lobe through an atypical autocrine/paracrine mechanism in addition to its role in axon guidance, and in association with proteins of the endocytic pathway and small GTPases.

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