CIRCULATING MIR-132, MIR-146A, MIR-222, AND MIR-320 EXPRESSION IN DIFFERENTIAL DIAGNOSIS OF WOMEN WITH POLYCYSTIC OVARY SYNDROME

Overview

Journal Acta Endocrinol (Buchar)

Specialty Endocrinology

Date 2022 Aug 17

PMID 35975247

Authors

Z Soyman

S Durmus

S Ates

G Simsek

V Sozer

B P Kundaktepe

D Kurtulus

R Gelisgen

V Sal

H Uzun

Affiliations

Soon will be listed here.

Abstract

Purpose: The aim of the study was to investigate whether the circulating miR-132, miR-146a, miR-222, and miR-320 levels are used in the differential diagnosis of women with polycystic ovary syndrome (PCOS) and healthy women.

Methods: This prospective case-control study included 50 women with PCOS and age- and body mass index- matched 50 healthy controls. The hormone and lipid profiles, levels of microRNAs (miRNAs), and parameters of carbohydrate metabolism were measured.

Results: Expression levels of miRNAs were assessed using the two-step quantitative real-time polymerase chain reaction. Circulating miR-132, miR-146a and miR-222 levels were significantly downregulated in the PCOS group compared with the control group. The miR-320 levels did not differ between the two groups. Free testosterone was negatively correlated with miR-132, miR-146a and miR-222. Insulin was negatively correlated with miR-132 and miR-146a.

Conclusions: The results of the study revealed that miRNA expression, may suggest a possible distinction between healthy women and PCOS patients. miR-132, miR-146a, and miR-222 may have key functions in the pathogenesis of PCOS.

Citing Articles

MicroRNAs as Biomarkers for Metabolic Disorders in Polycystic Ovary Syndrome (PCOS): A Review.

Dymanowska I, Frankowska K, Krawczyk A, Kociuba J, Gil-Kulik P, Banaszewska B Med Sci Monit. 2024; 30:e946480.

PMID: 39709519 PMC: 11670786. DOI: 10.12659/MSM.946480.

EXPRESSION PROFILES OF MIR-155-5P AND MIR-518B MICRORNAS IN CIRCULATING LEUKOCYTES OF THE PREGNANT PATIENTS WITH PREECLAMPSIA AND POLYCYSTIC OVARY SYNDROME.

Hocaoglu M, Demirer S, Karaalp I, Kaynak E, Attar E, Turgut A Acta Endocrinol (Buchar). 2024; 19(4):426-434.

PMID: 38933243 PMC: 11197839. DOI: 10.4183/aeb.2023.426.

Genetic variants in miR-146a and miR-196a2 in endometriosis: a Brazilian study.

Oliveira G, Cintra M, Lima M, Gomes M, Marqui A Rev Assoc Med Bras (1992). 2024; 70(5):e20231382.

PMID: 38775532 PMC: 11101185. DOI: 10.1590/1806-9282.20231382.

Decreased Serum Levels of the Insulin Resistance-Related microRNA miR-320a in Patients with Polycystic Ovary Syndrome.

Vogt S, Handke D, Behre H, Greither T Curr Issues Mol Biol. 2024; 46(4):3379-3393.

PMID: 38666942 PMC: 11049427. DOI: 10.3390/cimb46040212.

The Role of MicroRNA, Long Non-Coding RNA and Circular RNA in the Pathogenesis of Polycystic Ovary Syndrome: A Literature Review.

Nasser J, Altahoo N, Almosawi S, Alhermi A, Butler A Int J Mol Sci. 2024; 25(2).

PMID: 38255975 PMC: 10815174. DOI: 10.3390/ijms25020903.

References

Zhao C, Dong J, Jiang T, Shi Z, Yu B, Zhu Y . Early second-trimester serum miRNA profiling predicts gestational diabetes mellitus. PLoS One. 2011; 6(8):e23925. PMC: 3161072. DOI: 10.1371/journal.pone.0023925. View

Shi Y, Zhao H, Shi Y, Cao Y, Yang D, Li Z . Genome-wide association study identifies eight new risk loci for polycystic ovary syndrome. Nat Genet. 2012; 44(9):1020-5. DOI: 10.1038/ng.2384. View

Malini N, Roy G . Influence of Insulin on LH, Testosterone and SHBG in various PCOS Categories based on the Mode of Secretion of LH in relation to FSH Levels. Acta Endocrinol (Buchar). 2022; 17(3):313-318. PMC: 8919493. DOI: 10.4183/aeb.2021.313. View

Yin M, Deng Q, Li Y, Wang X, Li H . Compact and broadband mode multiplexer and demultiplexer based on asymmetric plasmonic-dielectric coupling. Appl Opt. 2014; 53(27):6175-80. DOI: 10.1364/AO.53.006175. View

Ortega F, Mercader J, Moreno-Navarrete J, Rovira O, Guerra E, Esteve E . Profiling of circulating microRNAs reveals common microRNAs linked to type 2 diabetes that change with insulin sensitization. Diabetes Care. 2014; 37(5):1375-83. DOI: 10.2337/dc13-1847. View

Asuncion M, Calvo R, San Millan J, Sancho J, Avila S, Escobar-Morreale H . A prospective study of the prevalence of the polycystic ovary syndrome in unselected Caucasian women from Spain. J Clin Endocrinol Metab. 2000; 85(7):2434-8. DOI: 10.1210/jcem.85.7.6682. View

Rossi J . New hope for a microRNA therapy for liver cancer. Cell. 2009; 137(6):990-2. DOI: 10.1016/j.cell.2009.05.038. View

Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K . Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 2008; 18(10):997-1006. DOI: 10.1038/cr.2008.282. View

Sang Q, Yao Z, Wang H, Feng R, Wang H, Zhao X . Identification of microRNAs in human follicular fluid: characterization of microRNAs that govern steroidogenesis in vitro and are associated with polycystic ovary syndrome in vivo. J Clin Endocrinol Metab. 2013; 98(7):3068-79. DOI: 10.1210/jc.2013-1715. View

10.

Balasubramanyam M, Aravind S, Gokulakrishnan K, Prabu P, Sathishkumar C, Ranjani H . Impaired miR-146a expression links subclinical inflammation and insulin resistance in Type 2 diabetes. Mol Cell Biochem. 2011; 351(1-2):197-205. DOI: 10.1007/s11010-011-0727-3. View

11.

Li S, Zhu J, Zhang W, Chen Y, Zhang K, Popescu L . Signature microRNA expression profile of essential hypertension and its novel link to human cytomegalovirus infection. Circulation. 2011; 124(2):175-84. DOI: 10.1161/CIRCULATIONAHA.110.012237. View

12.

Ebrahimi S, Reiisi S, Parchami Barjui S . Increased risk of polycystic ovary syndrome (PCOS) associated with CC genotype of miR-146a gene variation. Gynecol Endocrinol. 2018; 34(9):793-797. DOI: 10.1080/09513590.2018.1460341. View

13.

Ascaso J, Pardo S, Real J, Lorente R, Priego A, Carmena R . Diagnosing insulin resistance by simple quantitative methods in subjects with normal glucose metabolism. Diabetes Care. 2003; 26(12):3320-5. DOI: 10.2337/diacare.26.12.3320. View

14.

Sirotkin A, Ovcharenko D, Grossmann R, Laukova M, Mlyncek M . Identification of microRNAs controlling human ovarian cell steroidogenesis via a genome-scale screen. J Cell Physiol. 2009; 219(2):415-20. DOI: 10.1002/jcp.21689. View

15.

Gilbert E, Tay C, Hiam D, Teede H, Moran L . Comorbidities and complications of polycystic ovary syndrome: An overview of systematic reviews. Clin Endocrinol (Oxf). 2018; 89(6):683-699. DOI: 10.1111/cen.13828. View

16.

Flowers E, Aouizerat B, Abbasi F, Lamendola C, Grove K, Fukuoka Y . Circulating microRNA-320a and microRNA-486 predict thiazolidinedione response: Moving towards precision health for diabetes prevention. Metabolism. 2015; 64(9):1051-9. PMC: 4546550. DOI: 10.1016/j.metabol.2015.05.013. View

17.

Salimi-Asl M, Mozdarani H, Kadivar M . Up-regulation of miR-21 and 146a expression and increased DNA damage frequency in a mouse model of polycystic ovary syndrome (PCOS). Bioimpacts. 2016; 6(2):85-91. PMC: 4981253. DOI: 10.15171/bi.2016.12. View

18.

Ferriman D, GALLWEY J . Clinical assessment of body hair growth in women. J Clin Endocrinol Metab. 1961; 21:1440-7. DOI: 10.1210/jcem-21-11-1440. View

19.

Long W, Zhao C, Ji C, Ding H, Cui Y, Guo X . Characterization of serum microRNAs profile of PCOS and identification of novel non-invasive biomarkers. Cell Physiol Biochem. 2014; 33(5):1304-15. DOI: 10.1159/000358698. View

20.

Murri M, Insenser M, Fernandez-Duran E, San-Millan J, Luque-Ramirez M, Escobar-Morreale H . Non-targeted profiling of circulating microRNAs in women with polycystic ovary syndrome (PCOS): effects of obesity and sex hormones. Metabolism. 2018; 86:49-60. DOI: 10.1016/j.metabol.2018.01.011. View