Membrane Progesterone Receptor α (mPRα/PAQR7) Promotes Survival and Neurite Outgrowth of Human Neuronal Cells by a Direct Action and Through Schwann Cell-like Stem Cells

Overview

Journal J Mol Neurosci

Specialties Molecular Biology
Neurology

Date 2022 Aug 16

PMID 35974286

Authors

Luca F Castelnovo

Peter Thomas

Affiliations

Soon will be listed here.

Abstract

We recently showed that membrane progesterone receptor α (mPRα/PAQR7) promotes pro-regenerative effects in Schwann cell-like adipose stem cells (SCL-ASC), an alternative model to Schwann cells for the promotion of peripheral nerve regeneration. In this study, we investigated how mPRα activation with the mPR-specific agonist Org OD 02-0 in SCL-ASC affected regenerative parameters in two neuronal cell lines, IMR-32 and SH-SY-5Y. In a series of conditioned medium experiments, we found that mPR activation of SCL-ASC led to increased neurite outgrowth, protection from cell death and increased expression of peripheral nerve regeneration markers (CREB3, ATF3, GAP43) in neuronal cell lines. These effects were stronger than the ones observed with the conditioned medium from untreated SCL-ASC. The addition of Org OD 02-0 to the untreated cell medium mimicked the effects of mPR activation of SCL-ASC on cell death, but not on neurite outgrowth. Therefore, the effect of Org OD 02-0 on neurite outgrowth is SCL-ASC-dependent, while its effect on cell survivability is likely due to the direct activation of mPRs on neuronal cells. SCL-ASC transfection with mPRα siRNA showed that this isoform is responsible for the beneficial effect on neurite outgrowth. Further experiments showed that SCL-ASC-dependent outcomes likely involved the release of BDNF and IGF-2 from these cells. The beneficial mPRα effect on neurite outgrowth was confirmed in co-culture conditions. These findings strengthen the hypothesis that mPRα could play a pro-regenerative role in SCL-ASC and be a therapeutic target for the promotion of peripheral nerve regeneration.

Citing Articles

Brain-derived neuerotrophic factor and related mechanisms that mediate and influence progesterone-induced neuroprotection.

Singh M, Krishnamoorthy V, Kim S, Khurana S, LaPorte H Front Endocrinol (Lausanne). 2024; 15:1286066.

PMID: 38469139 PMC: 10925611. DOI: 10.3389/fendo.2024.1286066.

Membrane Progesterone Receptors (mPRs/PAQRs) Are Going beyond Its Initial Definitions.

Aickareth J, Hawwar M, Sanchez N, Gnanasekaran R, Zhang J Membranes (Basel). 2023; 13(3).

PMID: 36984647 PMC: 10056622. DOI: 10.3390/membranes13030260.

Progesterone exerts a neuroprotective action in a Parkinson's disease human cell model through membrane progesterone receptor α (mPRα/PAQR7).

Castelnovo L, Thomas P Front Endocrinol (Lausanne). 2023; 14:1125962.

PMID: 36967764 PMC: 10036350. DOI: 10.3389/fendo.2023.1125962.

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