» Articles » PMID: 35972547

Prevalence and Genetic Analysis of Enterobius Vermicularis in Schoolchildren in Lower Northern Thailand

Overview
Journal Parasitol Res
Specialty Parasitology
Date 2022 Aug 16
PMID 35972547
Authors
Affiliations
Soon will be listed here.
Abstract

Enterobius vermicularis, a nematode parasite with a global distribution causes enterobiasis in schoolchildren and is considered a neglected parasite. An understanding of the prevalence and genetic diversity of enterobiasis is crucial for appropriate control measures. Therefore, the objectives of this research were to study the prevalence and genetic diversity of E. vermicularis in schoolchildren from lower northern Thailand, based on cytochrome c oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2) sequences. Using the scotch tape technique, 7.4% (188/2544) of schoolchildren from 21 primary schools were found positive for E. vermicularis eggs, which is a relatively low infection rate. Phylogenetic trees of partial COI sequences (397 bp) revealed similar topologies using maximum likelihood (ML) and neighbor-joining (NJ) methods and identified E. vermicularis type A (105 sequences) and B (1 sequence). Haplotype network analysis of the COI sequences demonstrated a high haplotype diversity (H = 0.9028). In contrast, phylogenetic analysts of a 343 bp region of the ITS2 locus (52 sequences) revealed a monophyletic group. More sequence analyses of E. vermicularis from humans and other hosts in Thailand are necessary to better understand the genetic diversity of this parasite.

Citing Articles

Global prevalence of enterobiasis in young children over the past 20 years: a systematic review and meta-analysis.

Kia Lashaki E, Mizani A, Hosseini S, Habibi B, Taherkhani K, Javadi A Osong Public Health Res Perspect. 2024; 14(6):441-450.

PMID: 38204424 PMC: 10788413. DOI: 10.24171/j.phrp.2023.0204.

References
1.
Ariyarathenam A, Nachimuthu S, Tang T, Courtney E, Harris S, Harris A . Enterobius vermicularis infestation of the appendix and management at the time of laparoscopic appendectomy: case series and literature review. Int J Surg. 2010; 8(6):466-9. DOI: 10.1016/j.ijsu.2010.06.007. View

2.
Blouin M . Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. Int J Parasitol. 2002; 32(5):527-31. DOI: 10.1016/s0020-7519(01)00357-5. View

3.
Boas H, Tapia G, Rasmussen T, Ronningen K . Enterobius vermicularis and allergic conditions in Norwegian children. Epidemiol Infect. 2013; 142(10):2114-20. PMC: 9161229. DOI: 10.1017/S0950268813003154. View

4.
Burkhart C, Burkhart C . Assessment of frequency, transmission, and genitourinary complications of enterobiasis (pinworms). Int J Dermatol. 2005; 44(10):837-40. DOI: 10.1111/j.1365-4632.2004.02332.x. View

5.
Chai J, Yang S, Kim J, Choi S, Song G, Jung B . High Prevalence of Enterobius vermicularis Infection among Schoolchildren in Three Townships around Yangon, Myanmar. Korean J Parasitol. 2016; 53(6):771-5. PMC: 4725233. DOI: 10.3347/kjp.2015.53.6.771. View