Structural Characterization of a Hua Tuber Polysaccharide and Its Contribution to Moisture Retention and Moisture-Proofing of Porous Carbohydrate Material

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Aug 12

PMID 35956965

Authors

Ling Yu

Yipeng Wang

Qingjiu Tang

Rongrong Zhang

Danyu Zhang

Guangyong Zhu

Affiliations

Soon will be listed here.

Abstract

Porous carbohydrate materials such as tobacco shreds readily absorb moisture and become damp during processing, storage, and consumption (smoking). Traditional humectants have the ability of moisture retention but moisture-proofing is poor. Hua polysaccharide (PCP 85-1-1) was separated by fractional precipitation and was purified by anion exchange and gel permeation chromatography. The average molecular weight (Mw) of PCP 85-1-1 was 2.88 × 10 Da. The monosaccharide composition implied that PCP 85-1-1 consisted of fucose, glucose, and fructose, and the molar ratio was 22.73:33.63:43.65. When 2% PCP 85-1-1 was added to tobacco shreds, the ability of moisture retention and moisture-proofing were significantly enhanced. The moisture retention index (MRI) and moisture-proofing index (MPI) increased from 1.95 and 1.67 to 2.11 and 2.14, respectively. Additionally, the effects of PCP 85-1-1 on the aroma and taste of tobacco shreds were evaluated by electronic tongue and gas chromatography-mass spectrometry (GC-MS). These results indicated that PCP 85-1-1 had the characteristics of preventing water absorption under high relative humidity and moisturizing under dry conditions. The problem that traditional humectants are poorly moisture-proof was solved. PCP 85-1-1 can be utilized as a natural humectant on porous carbohydrates, which provides a reference for its development and utilization.

Citing Articles

Analysis of Water Adsorption Capacity, Thermal Behavior, Antioxidant Activity, and Smoke Volume by Polyol Ester in Reconstituted Tobacco.

Tian H, Gao Z, Han L, Wan J, Yang X, Li M ACS Omega. 2024; 9(44):44336-44346.

PMID: 39524655 PMC: 11541444. DOI: 10.1021/acsomega.4c05199.

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants.

Xu M, Hu J, Li H, Li K, Xu D PeerJ. 2024; 12:e17052.

PMID: 38464751 PMC: 10924778. DOI: 10.7717/peerj.17052.

References

He L, Yan B, Yao C, Chen X, Li L, Wu Y . Oligosaccharides from Polygonatum Cyrtonema Hua: Structural characterization and treatment of LPS-induced peritonitis in mice. Carbohydr Polym. 2021; 255:117392. DOI: 10.1016/j.carbpol.2020.117392. View

Wang Y, Liu N, Xue X, Li Q, Sun D, Zhao Z . Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum. Int J Biol Macromol. 2020; 160:688-694. DOI: 10.1016/j.ijbiomac.2020.05.245. View

Zhao P, Li X, Wang Y, Zhang X, Jia H, Guo L . Comparative studies on characterization, saccharide mapping and antiglycation activity of polysaccharides from different Polygonatum ssp. J Pharm Biomed Anal. 2020; 186:113243. DOI: 10.1016/j.jpba.2020.113243. View

Long T, Liu Z, Shang J, Zhou X, Yu S, Tian H . Polygonatum sibiricum polysaccharides play anti-cancer effect through TLR4-MAPK/NF-κB signaling pathways. Int J Biol Macromol. 2018; 111:813-821. DOI: 10.1016/j.ijbiomac.2018.01.070. View

Li J, Wang W, Zheng G, Li L . Physicochemical properties and antioxidant activities of polysaccharides from Gynura procumbens leaves by fractional precipitation. Int J Biol Macromol. 2016; 95:719-724. DOI: 10.1016/j.ijbiomac.2016.11.113. View

Pu Y, Liu Z, Zhong C, Zhang X, Bao Y . Immunomodulatory effects of a polysaccharide from Solanum nigrum Linne through TLR4-MyD88 signaling pathway. Int Immunopharmacol. 2020; 88:106973. DOI: 10.1016/j.intimp.2020.106973. View

Qiu Y, Ma Y, Huang Y, Li S, Xu H, Su E . Current advances in the biosynthesis of hyaluronic acid with variable molecular weights. Carbohydr Polym. 2021; 269:118320. DOI: 10.1016/j.carbpol.2021.118320. View

Gong G, Dang T, Deng Y, Han J, Zou Z, Jing S . Physicochemical properties and biological activities of polysaccharides from Lycium barbarum prepared by fractional precipitation. Int J Biol Macromol. 2017; 109:611-618. DOI: 10.1016/j.ijbiomac.2017.12.017. View

Liu F, Liu Y, Meng Y, Yang M, He K . Structure of polysaccharide from Polygonatum cyrtonema Hua and the antiherpetic activity of its hydrolyzed fragments. Antiviral Res. 2004; 63(3):183-9. DOI: 10.1016/j.antiviral.2004.04.006. View

10.

Schepetkin I, Quinn M . Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int Immunopharmacol. 2006; 6(3):317-33. DOI: 10.1016/j.intimp.2005.10.005. View

11.

Shao P, Shao J, Han L, Lv R, Sun P . Separation, preliminary characterization, and moisture-preserving activity of polysaccharides from Ulva fasciata. Int J Biol Macromol. 2014; 72:924-30. DOI: 10.1016/j.ijbiomac.2014.09.048. View

12.

Wang L, Cardenas R, Watson C . An isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sugars and humectants in tobacco products. J Chromatogr A. 2017; 1514:95-102. PMC: 5695665. DOI: 10.1016/j.chroma.2017.07.079. View

13.

Talhout R, Opperhuizen A, van Amsterdam J . Sugars as tobacco ingredient: Effects on mainstream smoke composition. Food Chem Toxicol. 2006; 44(11):1789-98. DOI: 10.1016/j.fct.2006.06.016. View

14.

Sun T, Zhang H, Li Y, Liu Y, Dai W, Fang J . Physicochemical properties and immunological activities of polysaccharides from both crude and wine-processed Polygonatum sibiricum. Int J Biol Macromol. 2019; 143:255-264. DOI: 10.1016/j.ijbiomac.2019.11.166. View

15.

Zhang H, Cai X, Tian Q, Xiao L, Zeng Z, Cai X . Microwave-Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity. J Food Sci. 2019; 84(4):754-761. DOI: 10.1111/1750-3841.14449. View

16.

Lv X, Chen D, Yang L, Zhu N, Li J, Zhao J . Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system. Int J Biol Macromol. 2016; 86:28-42. DOI: 10.1016/j.ijbiomac.2016.01.048. View

17.

Li R, Tao A, Yang R, Fan M, Zhang X, Du Z . Structural characterization, hypoglycemic effects and antidiabetic mechanism of a novel polysaccharides from Polygonatum kingianum Coll. et Hemsl. Biomed Pharmacother. 2020; 131:110687. DOI: 10.1016/j.biopha.2020.110687. View

18.

Liu N, Dong Z, Zhu X, Xu H, Zhao Z . Characterization and protective effect of Polygonatum sibiricum polysaccharide against cyclophosphamide-induced immunosuppression in Balb/c mice. Int J Biol Macromol. 2017; 107(Pt A):796-802. DOI: 10.1016/j.ijbiomac.2017.09.051. View

19.

Chou C, Sung T, Hu Y, Lu H, Yang L, Cheng K . Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation. Int J Biol Macromol. 2019; 131:1021-1031. DOI: 10.1016/j.ijbiomac.2019.03.154. View

20.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View