A Two-Photon Fluorescent Probe for the Visual Detection of Peroxynitrite in Living Cells and Zebrafish

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Aug 12

PMID 35956806

Authors

Zhencai Xu

Jin Qian

Yufeng Ge

Yalan Wang

Hongwei Chen

Affiliations

Soon will be listed here.

Abstract

Peroxynitrite (ONOO), as an important reactive oxygen species (ROS), holds great potential to react with a variety of biologically active substances, leading to the occurrence of various diseases such as cancer and neurodegenerative diseases. In this work, we developed a novel mitochondria-localized fluorescent probe, HDBT-ONOO, which was designed as a mitochondria-targeting two-photon fluorescence probe based on 1,8-naphthylimide fluorophore and the reactive group of 4-(bromomethyl)-benzene boronic acid pinacol ester. More importantly, the probe exhibited good biocompatibility, sensitivity, and selectivity, enabling its successful application in imaging the generation of intracellular and extracellular ONOO. Furthermore, exogenous and endogenous ONOO products in live zebrafish were visualized. It is greatly expected that the designed probe can serve as a useful imaging tool for clarifying the distribution and pathophysiological functions of ONOO in cells and zebrafish.

Citing Articles

Role of Peroxynitrite in the Pathogenesis of Parkinson's Disease and Its Fluorescence Imaging-Based Detection.

Lv J, Chen F, Zhang C, Kang Y, Yang Y, Zhang C Biosensors (Basel). 2024; 14(10).

PMID: 39451719 PMC: 11506598. DOI: 10.3390/bios14100506.

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