Higher Loading of Gold Nanoparticles in PAD Mesenchymal-like Stromal Cells Leads to a Decreased Exocytosis

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 Aug 12

PMID 35954168

Authors

Jennifer Oberlander

Rafael Ayerbe

Joan Cabellos

Richard da Costa Marques

Bin Li

Nazende Gunday-Tureli

Akif Emre Tureli

Racheli Ofir

Eliran Ish Shalom

Volker Mailander

Affiliations

Soon will be listed here.

Abstract

Cell therapy is an important new method in medicine and is being used for the treatment of an increasing number of diseases. The challenge here is the precise tracking of cells in the body and their visualization. One method to visualize cells more easily with current methods is their labeling with nanoparticles before injection. However, for a safe and sufficient cell labeling, the nanoparticles need to remain in the cell and not be exocytosed. Here, we test a glucose-PEG-coated gold nanoparticle for the use of such a cell labeling. To this end, we investigated the nanoparticle exocytosis behavior from PLX-PAD cells, a cell type currently in clinical trials as a potential therapeutic agent. We showed that the amount of exocytosed gold from the cells was influenced by the uptake time and loading amount. This observation will facilitate the safe labeling of cells with nanoparticles in the future and contribute to stem cell therapy research.

Citing Articles

Biological Performance of Primary Dental Pulp Stem Cells Treated with Gold Nanoparticles.

Abuarqoub D, Mahmoud N, Alshaer W, Mohammad M, Ibrahim A, Al-Mrahleh M Biomedicines. 2023; 11(9).

PMID: 37760931 PMC: 10525781. DOI: 10.3390/biomedicines11092490.

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