Polysaccharides of Weissella Cibaria Act As a Prebiotic to Enhance the Probiotic Potential of Lactobacillus Rhamnosus

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2022 Aug 10

PMID 35947292

Authors

Soyoung Park

Kandasamy Saravanakumar

Anbazhagan Sathiyaseelan

Ki-Seok Han

Jooeun Lee

Myeong-Hyeon Wang

Affiliations

Soon will be listed here.

Abstract

This work aimed to investigate the effect of EPS (extracellular polysaccharide) of Weissella cibaria as a prebiotic to promote the growth and antibacterial properties of Lactobacillus rhamnosus. The morphological, growth behavior, and antibacterial properties of L. rhamnosus were determined in MRSB (de Man Rogosa Sharpe broth) supplemented with different concentrations of EPS (0.1-2%). The results revealed that the incorporation of the EPS (2%) in MRSA improved the bacterial growth in terms of colony-forming unit (CFU, 0.7 × 10 CFU/mL) compared to L. rhamnosus grown in bare MRSA. The SEM observation revealed that EPS incorporation in the MRSB culture media does not affect the morphological properties of L. rhamnosus. Moreover, it was confirmed that the extract of probiotics cultured in MRSA supplemented with EPS (2%) was exhibited strong antibacterial and antibiofilm activity against targeted pathogens. This L. rhamnosus extract was found to be biocompatible evidanced by erythrocyte hemolysis assay. These results confirmed that EPS regulates the growth of probiotics, resists pathogen infection, and biocompatibility.

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