Insulin-like Growth Factor Binding Protein 5 Accelerate the Senescence of Periodontal Ligament Stem Cells

Overview

Journal Cell Tissue Bank

Specialties Anatomy & Histology
Cell Biology
General Surgery

Date 2022 Aug 8

PMID 35939161

Authors

Le Li

Haiyan Wang

Dianqin Yu

Yantong Wang

Yingchu Zhao

Haoqing Yang

Yangyang Cao

Yu Cao

Affiliations

Soon will be listed here.

Abstract

Evidences have showed stem cell mediated tissue regeneration is a promising method for the treatment of periodontitis. Insulin-like growth factor binding proteins-5 (IGFBP5) is a member of the insulin growth factor (IGFs) family and plays a regulatory role in cell proliferation and differentiation. Our previous study showed that IGFBP5 can promote osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and enhance periodontal tissue regeneration mediated by PDLSCs. However, the function of IGFBP5 in the process of PDLSCs senescence remains unclear. The present study showed IGFBP5 mRNA level was highly expressed in passage-induced aged PDLSCs cells. IGFBP5 knockdown decreased the ratio of senescence associated β-galactosidase (SA-β-Gal) positive cells, enhanced the activity of TERT, and down-regulated the expression levels of P16, P21, P53 mRNA and protein. Overexpression of IGFBP5 increased the ratio of SA-β-Gal positive staining PDLSCs, decreased the activity of telomerase TERT, and up-regulated the expression levels of P16, P21, P53 mRNA and protein related to PDLSCs senescence. In conclusion, IGFBP5 can accelerate the senescence of PDLSCs, indicating the potential target for maintaining the "young state" of stem cells.

Citing Articles

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