Recent Advances in Nanotechnology-enabled Biosensors for Detection of Exosomes As New Cancer Liquid Biopsy

Overview

Journal Exp Biol Med (Maywood)

Specialty Biology

Date 2022 Aug 8

PMID 35938477

Authors

Chang-Chieh Hsu

Yun Wu

Affiliations

Soon will be listed here.

Abstract

Cancer liquid biopsy detects circulating biomarkers in body fluids, provides information that complements medical imaging and tissue biopsy, allows sequential monitoring of cancer development, and, therefore, has shown great promise in cancer screening, diagnosis, and prognosis. Exosomes (also known as small extracellular vesicles) are cell-secreted, nanosized vesicles that transport biomolecules such as proteins and RNAs for intercellular communication. Exosomes are actively involved in cancer development and progression and have become promising circulating biomarkers for cancer liquid biopsy. Conventional exosome characterization methods such as quantitative reverse transcription polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) are limited by low sensitivity, tedious process, large sample volume, and high cost. To overcome these challenges, new biosensors have been developed to offer sensitive, simple, fast, high throughput, low sample consumption, and cost-effective detection of exosomal biomarkers. In this review, we summarized recent advances in nanotechnology-enabled biosensors that detect exosomal RNAs (both microRNAs and mRNAs) and proteins for cancer screening, diagnosis, and prognosis. The biosensors were grouped based on their sensing mechanisms, including fluorescence-based biosensors, colorimetric biosensors, electrical/electrochemical biosensors, plasmonics-based biosensors, surface-enhanced Raman spectroscopy (SERS)-based biosensors, and inductively coupled plasma mass spectrometry (ICP-MS) and photothermal biosensors. The future directions for the development of exosome-based biosensors were discussed.

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References

Zhu K, Wang Z, Zong S, Liu Y, Yang K, Li N . Hydrophobic Plasmonic Nanoacorn Array for a Label-Free and Uniform SERS-Based Biomolecular Assay. ACS Appl Mater Interfaces. 2020; 12(26):29917-29927. DOI: 10.1021/acsami.0c03993. View

Dong H, Chen H, Jiang J, Zhang H, Cai C, Shen Q . Highly Sensitive Electrochemical Detection of Tumor Exosomes Based on Aptamer Recognition-Induced Multi-DNA Release and Cyclic Enzymatic Amplification. Anal Chem. 2018; 90(7):4507-4513. DOI: 10.1021/acs.analchem.7b04863. View

Xia Y, Liu M, Wang L, Yan A, He W, Chen M . A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes. Biosens Bioelectron. 2017; 92:8-15. DOI: 10.1016/j.bios.2017.01.063. View

Wu X, Zhao H, Natalia A, Lim C, Ho N, Ong C . Exosome-templated nanoplasmonics for multiparametric molecular profiling. Sci Adv. 2020; 6(19):eaba2556. PMC: 7202874. DOI: 10.1126/sciadv.aba2556. View

Zhang P, Zhou X, He M, Shang Y, Tetlow A, Godwin A . Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip. Nat Biomed Eng. 2019; 3(6):438-451. PMC: 6556143. DOI: 10.1038/s41551-019-0356-9. View

Pu X, Ding G, Wu M, Zhou S, Jia S, Cao L . Elevated expression of exosomal microRNA-21 as a potential biomarker for the early diagnosis of pancreatic cancer using a tethered cationic lipoplex nanoparticle biochip. Oncol Lett. 2020; 19(3):2062-2070. PMC: 7039151. DOI: 10.3892/ol.2020.11302. View

Wang X, Kwak K, Yang Z, Zhang A, Zhang X, Sullivan R . Extracellular mRNA detected by molecular beacons in tethered lipoplex nanoparticles for diagnosis of human hepatocellular carcinoma. PLoS One. 2018; 13(6):e0198552. PMC: 5991670. DOI: 10.1371/journal.pone.0198552. View

Liu C, Kannisto E, Yu G, Yang Y, Reid M, Patnaik S . Non-invasive Detection of Exosomal MicroRNAs via Tethered Cationic Lipoplex Nanoparticles (tCLN) Biochip for Lung Cancer Early Detection. Front Genet. 2020; 11:258. PMC: 7100709. DOI: 10.3389/fgene.2020.00258. View

Yang Y, Kannisto E, Patnaik S, Reid M, Li L, Wu Y . Ultrafast Detection of Exosomal RNAs Cationic Lipoplex Nanoparticles in a Micromixer Biochip for Cancer Diagnosis. ACS Appl Nano Mater. 2021; 4(3):2806-2819. PMC: 8628515. DOI: 10.1021/acsanm.0c03426. View

10.

He D, Wang H, Ho S, Chan H, Hai L, He X . Total internal reflection-based single-vesicle in situ quantitative and stoichiometric analysis of tumor-derived exosomal microRNAs for diagnosis and treatment monitoring. Theranostics. 2019; 9(15):4494-4507. PMC: 6599656. DOI: 10.7150/thno.33683. View

11.

Huang R, He L, Li S, Liu H, Jin L, Chen Z . A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification. Nanoscale. 2020; 12(4):2445-2451. DOI: 10.1039/c9nr08747h. View

12.

Cao Y, Wang Y, Yu X, Jiang X, Li G, Zhao J . Identification of programmed death ligand-1 positive exosomes in breast cancer based on DNA amplification-responsive metal-organic frameworks. Biosens Bioelectron. 2020; 166:112452. DOI: 10.1016/j.bios.2020.112452. View

13.

Kwizera E, OConnor R, Vinduska V, Williams M, Butch E, Snyder S . Molecular Detection and Analysis of Exosomes Using Surface-Enhanced Raman Scattering Gold Nanorods and a Miniaturized Device. Theranostics. 2018; 8(10):2722-2738. PMC: 5957005. DOI: 10.7150/thno.21358. View

14.

Wu W, Yu X, Wu J, Wu T, Fan Y, Chen W . Surface plasmon resonance imaging-based biosensor for multiplex and ultrasensitive detection of NSCLC-associated exosomal miRNAs using DNA programmed heterostructure of Au-on-Ag. Biosens Bioelectron. 2020; 175:112835. DOI: 10.1016/j.bios.2020.112835. View

15.

Moller A, Lobb R . The evolving translational potential of small extracellular vesicles in cancer. Nat Rev Cancer. 2020; 20(12):697-709. DOI: 10.1038/s41568-020-00299-w. View

16.

Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F . ExoAPP: Exosome-Oriented, Aptamer Nanoprobe-Enabled Surface Proteins Profiling and Detection. Anal Chem. 2018; 90(24):14402-14411. DOI: 10.1021/acs.analchem.8b03959. View

17.

Tsang D, Lieberthal T, Watts C, Dunlop I, Ramadan S, Del Rio Hernandez A . Chemically Functionalised Graphene FET Biosensor for the Label-free Sensing of Exosomes. Sci Rep. 2019; 9(1):13946. PMC: 6763426. DOI: 10.1038/s41598-019-50412-9. View

18.

Zhou B, Xu K, Zheng X, Chen T, Wang J, Song Y . Application of exosomes as liquid biopsy in clinical diagnosis. Signal Transduct Target Ther. 2020; 5(1):144. PMC: 7400738. DOI: 10.1038/s41392-020-00258-9. View

19.

Li B, Pan W, Liu C, Guo J, Shen J, Feng J . Homogenous Magneto-Fluorescent Nanosensor for Tumor-Derived Exosome Isolation and Analysis. ACS Sens. 2020; 5(7):2052-2060. DOI: 10.1021/acssensors.0c00513. View

20.

Joshi G, Deitz-McElyea S, Liyanage T, Lawrence K, Mali S, Sardar R . Label-Free Nanoplasmonic-Based Short Noncoding RNA Sensing at Attomolar Concentrations Allows for Quantitative and Highly Specific Assay of MicroRNA-10b in Biological Fluids and Circulating Exosomes. ACS Nano. 2015; 9(11):11075-89. PMC: 4660391. DOI: 10.1021/acsnano.5b04527. View