Sex Chromosome Aneuploidies Give Rise to Changes in the Circular RNA Profile: A Circular Transcriptome-wide Study of Turner and Klinefelter Syndrome Across Different Tissues

Overview

Journal Front Genet

Date 2022 Aug 8

PMID 35938026

Authors

Emma B Johannsen

Jesper Just

Mette H Viuff

Trine Line Hauge Okholm

Steen B Pedersen

Katrine Meyer Lauritsen

Christian Trolle

Mette Glavind Bulow Pedersen

Simon Chang

Jens Fedder

Anne Skakkebaek

Claus H Gravholt

Affiliations

Soon will be listed here.

Abstract

The landscape of circular RNAs (circRNAs), an important class of non-coding RNAs that regulate gene expression, has never been described in human disorders of sex chromosome aneuploidies. We profiled circRNAs in Turner syndrome females (45,X; TS) and Klinefelter syndrome males (47,XXY; KS) to investigate how circRNAs respond to a missing or an extra X chromosome. Samples of blood, muscle and fat were collected from individuals with TS ( = 33) and KS ( = 22) and from male ( = 16) and female ( = 44) controls. CircRNAs were identified using a combination of circRNA identification pipelines (CIRI2, CIRCexplorer2 and circRNA_finder). Differential expression of circRNAs was observed throughout the genome in TS and KS, in all tissues. The host-genes from which several of these circRNAs were derived, were associated with known phenotypic traits. Furthermore, several differentially expressed circRNAs had the potential to capture micro RNAs that targeted protein-coding genes with altered expression in TS and KS. Sex chromosome aneuploidies introduce changes in the circRNA transcriptome, demonstrating that the genomic changes in these syndromes are more complex than hitherto thought. CircRNAs may help explain some of the genomic and phenotypic traits observed in these syndromes.

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