Effect of Guanosine Triphosphate on the Release and Uptake of Ca2+ in Saponin-permeabilized Macrophages and the Skeletal-muscle Sarcoplasmic Reticulum

Overview

Journal Biochem J

Specialty Biochemistry

Date 1987 Feb 15

PMID 3593238

Citations 6

Authors

T Hamachi

M Hirata

Y Kimura

T Ikebe

T Ishimatsu

K Yamaguchi

T Koga

Affiliations

Soon will be listed here.

Abstract

The effects of GTP, with or without polyethylene glycol (PEG), on the release and uptake of Ca2+ were examined by using saponin-treated macrophages and sarcoplasmic reticulum isolated from skeletal muscles. The application of GTP in concentrations in the range 0.1-10 microM induced a gradual, small but sustained release of Ca2+ from the saponin-treated macrophages. The addition of PEG to GTP markedly enhanced the GTP-mediated Ca2+ release. GTP at the same concentration ranges used for Ca2+ release decreased the amount of Ca2+ uptake, at a steady state, but stimulated the rate of Ca2+ accumulation in the presence of oxalate, the Ca2+-precipitating anion. The addition of PEG abolished the GTP-evoked stimulation of Ca2+ accumulation in the presence of oxalate. The stimulating effect on the rate of Ca2+ accumulation by GTP and its elimination by PEG were not due to changes in the permeability of oxalate by either GTP or PEG, or both. The Ca2+-releasing effect of GTP without PEG was enhanced by eliminating the uptake activity by decreasing the content of ATP. These results indicate that GTP has an inherent activity to release Ca2+ from non-mitochondrial intracellular stores of saponin-treated macrophages, and PEG enhances the GTP-mediated Ca2+ release, partly owing to its eliminating effect on GTP-stimulated Ca2+ uptake activity. These effects of GTP observed with saponin-permeabilized macrophages were not apparent in the isolated skeletal-muscle sarcoplasmic reticulum.

Citing Articles

Calcium entry into the inositol 1,4,5-trisphosphate-releasable calcium pool is mediated by a GTP-regulatory mechanism.

MULLANEY J, Yu M, Ghosh T, Gill D Proc Natl Acad Sci U S A. 1988; 85(8):2499-503.

PMID: 3357878 PMC: 280024. DOI: 10.1073/pnas.85.8.2499.

Possible physiological role of guanosine triphosphate and inositol 1,4,5-trisphosphate in Ca2+ release in macrophages stimulated with chemotactic peptide.

Kimura Y, Hirata M, Hamachi T, Koga T Biochem J. 1988; 249(2):531-6.

PMID: 3257693 PMC: 1148734. DOI: 10.1042/bj2490531.

GTP-mediated Ca2+ release in rough endoplasmic reticulum. Correlation with a GTP-sensitive increase in membrane permeability.

Nicchitta C, Joseph S, Williamson J Biochem J. 1987; 248(3):741-7.

PMID: 2829838 PMC: 1148612. DOI: 10.1042/bj2480741.

Relationship between hormonal, GTP and Ins(1,4,5)P3-stimulated Ca2+ uptake and release in pancreatic acinar cells.

Muallem S, Beeker T Biochem J. 1989; 263(2):333-9.

PMID: 2688630 PMC: 1133434. DOI: 10.1042/bj2630333.

Effects of guanosine nucleotides on skinned smooth muscle tissue of the rabbit mesenteric artery.

Fujiwara T, Itoh T, Kubota Y, Kuriyama H J Physiol. 1989; 408:535-47.

PMID: 2674422 PMC: 1190418. DOI: 10.1113/jphysiol.1989.sp017474.

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