Regional Glucose Uptake and Protein Synthesis in Isolated Perfused Rat Hearts Immediately After Training and Later

Overview

Journal Basic Res Cardiol

Specialty Cardiology & Vascular Diseases

Date 1987 Jan 1

PMID 3593186

Citations 2

Authors

H Kainulainen

J Komulainen

T Takala

V Vihko

Affiliations

Soon will be listed here.

Abstract

The effect of 10 weeks of running training and termination of training on the regional distribution of cardiac glucose uptake and protein synthesis were studied in isolated perfused hearts in male rats. The left ventricular glucose uptake in hearts from sedentary rats was 1.87 +/- 0.14 mumol/min per g protein (mean +/- SE), being about 30% higher in the subendocardial than in the subepicardial layer (p less than 0.05). The gradient of left ventricular glucose uptake was similar to the controls in the rats retired from training, but was absent in the trained animals. The altered transmural glucose uptake probably reflects differences in the adaptive response of various myocardial muscle layers to a long-term intermittent increase in cardiac work load. Phenylalanine incorporation was evenly distributed through the left ventricle in all the groups, but was lowered in the left and right ventricles of the trained rats. Phenylalanine incorporation returned to the control level 5 weeks after the cessation of training.

Citing Articles

Effect of chronic exercise on glucose uptake and activities of glycolytic enzymes measured regionally in rat heart.

Kainulainen H, Komulainen J, Takala T, Vihko V Basic Res Cardiol. 1989; 84(2):174-90.

PMID: 2730524 DOI: 10.1007/BF01907927.

Effects of training and anabolic steroids on collagen synthesis in dog heart.

Takala T, Ramo P, Kiviluoma K, Vihko V, Kainulainen H, Kettunen R Eur J Appl Physiol Occup Physiol. 1991; 62(1):1-6.

PMID: 1848813 DOI: 10.1007/BF00635624.

References

Sartore S, Gorza L, Pierobon Bormioli S, Dalla Libera L, Schiaffino S . Myosin types and fiber types in cardiac muscle. I. Ventricular myocardium. J Cell Biol. 1981; 88(1):226-33. PMC: 2111721. DOI: 10.1083/jcb.88.1.226. View

Morgan H, GORDON E, Kira Y, Siehl D, Watson P, Chua B . Wiggers Award lecture. Biochemical correlates of myocardial hypertrophy. Physiologist. 1985; 28(1):18-27. View

Nesher R, KARL I, KIPNIS D . Dissociation of effects of insulin and contraction on glucose transport in rat epitrochlearis muscle. Am J Physiol. 1985; 249(3 Pt 1):C226-32. DOI: 10.1152/ajpcell.1985.249.3.C226. View

Giusti R, Bersohn M, Malhotra A, Scheuer J . Cardiac function and actomyosin ATPase activity in hearts of conditioned and deconditioned rats. J Appl Physiol Respir Environ Exerc Physiol. 1978; 44(2):171-4. DOI: 10.1152/jappl.1978.44.2.171. View

Kobayashi K, Neely J . Control of maximum rates of glycolysis in rat cardiac muscle. Circ Res. 1979; 44(2):166-75. DOI: 10.1161/01.res.44.2.166. View

WHITTY A, Dimino M, Elfont E, Hughes G, Repeck M . Transmural mitochondrial differences in myocardium. Recent Adv Stud Cardiac Struct Metab. 1976; 11:349-54. View

CRASS 3rd M, McCaskill E, Shipp J . Glucose-free fatty acid interactions in the working heart. J Appl Physiol. 1970; 29(1):87-91. DOI: 10.1152/jappl.1970.29.1.87. View

Wallberg-Henriksson H, Holloszy J . Activation of glucose transport in diabetic muscle: responses to contraction and insulin. Am J Physiol. 1985; 249(3 Pt 1):C233-7. DOI: 10.1152/ajpcell.1985.249.3.C233. View

Olsson R . Changes in content of purine nucleoside in canine myocardium during coronary occlusion. Circ Res. 1970; 26(3):301-6. DOI: 10.1161/01.res.26.3.301. View

10.

Breull W, Flohr H, Schuchardt S, Dohm H . Transmural gradients in myocardial metabolic rate. Basic Res Cardiol. 1981; 76(4):399-403. DOI: 10.1007/BF01908331. View

11.

Dohm G, Beecher G, Hecker A, Puente F, Klain G, Askew E . Changes in protein synthesis in rats in response to endurance training. Life Sci. 1977; 21(2):189-97. DOI: 10.1016/0024-3205(77)90301-0. View

12.

Rupp H . The adaptive changes in the isoenzyme pattern of myosin from hypertrophied rat myocardium as a result of pressure overload and physical training. Basic Res Cardiol. 1981; 76(1):79-88. DOI: 10.1007/BF01908164. View

13.

Kainulainen H, Takala T, Hassinen I, Vihko V . Redistribution of glucose uptake by chronic exercise, measured in isolated perfused rat hearts. Pflugers Arch. 1985; 403(3):296-300. DOI: 10.1007/BF00583603. View

14.

Morady F, Laks M, Parmley W . Comparison of sarcomere lengths from normal and hypertrophied inner and middle canine right ventricle. Am J Physiol. 1973; 225(6):1257-9. DOI: 10.1152/ajplegacy.1973.225.6.1257. View

15.

Neely J, Morgan H . Relationship between carbohydrate and lipid metabolism and the energy balance of heart muscle. Annu Rev Physiol. 2009; 36:413-59. DOI: 10.1146/annurev.ph.36.030174.002213. View

16.

Hassinen I, Hiltunen K . Respiratory control in isolated perfused rat heart. Role of the equilibrium relations between the mitochondrial electron carriers and the adenylate system. Biochim Biophys Acta. 1975; 408(3):319-30. DOI: 10.1016/0005-2728(75)90133-4. View

17.

Anversa P, Loud A, Giacomelli F, Wiener J . Absolute morphometric study of myocardial hypertrophy in experimental hypertension. II. Ultrastructure of myocytes and interstitium. Lab Invest. 1978; 38(5):597-609. View

18.

Sokoloff L, Reivich M, Kennedy C, Des Rosiers M, PATLAK C, Pettigrew K . The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem. 1977; 28(5):897-916. DOI: 10.1111/j.1471-4159.1977.tb10649.x. View

19.

Takala T, Hassinen I . Effect of mechanical work load on the transmural distribution of glucose uptake in the isolated perfused rat heart, studied by regional deoxyglucose trapping. Circ Res. 1981; 49(1):62-9. DOI: 10.1161/01.res.49.1.62. View

20.

Everett A, Taylor R, Sparrow M . Protein synthesis during right-ventricular hypertrophy after pulmonary-artery stenosis in the dog. Biochem J. 1977; 166(3):315-21. PMC: 1165012. DOI: 10.1042/bj1660315. View