Comparative Evaluation of IL-17 and TGF-β Expression in Tissues of Patients with Chronic Periodontitis and Healthy Individuals Using Real-time PCR

Overview

Journal J Adv Periodontol Implant Dent

Specialty Dentistry

Date 2022 Aug 3

PMID 35919774

Authors

Kazem Fatemi

Seyed Abdolrahim Rezaee

Amir Moeintaghavi

Farid Shiezadeh

Golnaz Dadpour

Mohammad Rasoul Asadinezhad

Nahid Nasrabadi

Affiliations

Soon will be listed here.

Abstract

Background: The present study aimed to determine the association between periodontal disease and the Th17/Treg balance by examining the genetic expression of IL-17 and TGF-β, which influence incidence and suppression of inflammation.

Methods: In this case-control study, samples were collected in a randomized and task-oriented order. Thirty-seven patients referred to professional periodontology clinics in Mashhad and the Periodontology Department of the Mashhad Dentistry Faculty for periodontal (case) or crown-length (control) surgery was enrolled. IL-17 and TGF-β gene expression indices were measured in tissue samples by real-time polymerase chain reaction.

Results: The IL-17 gene expression index was higher in the case group (2.68±0.91) than in the control group (1.68±0.41), but this difference was not significant. The TGF-β gene expression index was significantly higher in the case group (54.42±7.88) than in the control group (24.12±3.38).

Conclusion: L-17 and TGF-β expression is increased in chronic periodontitis patients, but TGF-β plays a more important role in periodontal inflammation in patients with chronic periodontitis. Further studies of the roles of Th17 and Treg cells are warranted.

References

Shaker O, Ghallab N . IL-17 and IL-11 GCF levels in aggressive and chronic periodontitis patients: relation to PCR bacterial detection. Mediators Inflamm. 2012; 2012:174764. PMC: 3513783. DOI: 10.1155/2012/174764. View

Kadkhodazadeh M, Baghani Z, Ebadian A, Youssefi N, Mehdizadeh A, Azimi N . IL-17 gene polymorphism is associated with chronic periodontitis and peri-implantitis in Iranian patients: a cross-sectional study. Immunol Invest. 2013; 42(2):156-63. DOI: 10.3109/08820139.2012.746697. View

Khalaf H, Lonn J, Bengtsson T . Cytokines and chemokines are differentially expressed in patients with periodontitis: possible role for TGF-β1 as a marker for disease progression. Cytokine. 2014; 67(1):29-35. DOI: 10.1016/j.cyto.2014.02.007. View

Fu Q, Zhang L, Duan L, Qian S, Pang H . Correlation of chronic periodontitis in tropical area and IFN-γ, IL-10, IL-17 levels. Asian Pac J Trop Med. 2013; 6(6):489-92. DOI: 10.1016/S1995-7645(13)60080-2. View

Stevens R, Austen K . Recent advances in the cellular and molecular biology of mast cells. Immunol Today. 1989; 10(11):381-6. DOI: 10.1016/0167-5699(89)90272-7. View

Darveau R . Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010; 8(7):481-90. DOI: 10.1038/nrmicro2337. View

Qian B, Wahl S . TGF-beta can leave you breathless. Curr Opin Pharmacol. 2009; 9(4):454-61. PMC: 2730990. DOI: 10.1016/j.coph.2009.04.001. View

Gaffen S, Hajishengallis G . A new inflammatory cytokine on the block: re-thinking periodontal disease and the Th1/Th2 paradigm in the context of Th17 cells and IL-17. J Dent Res. 2008; 87(9):817-28. PMC: 2692983. DOI: 10.1177/154405910808700908. View

Sattari M, Fathiyeh A, Gholami F, Darbandi Tamijani H, Ghatreh Samani M . Effect of surgical flap on IL-1β and TGF-β concentrations in the gingival crevicular fluid of patients with moderate to severe chronic periodontitis. Iran J Immunol. 2011; 8(1):20-6. DOI: IJIv8i1A3. View

10.

Seymour G, Gemmell E . Cytokines in periodontal disease: where to from here?. Acta Odontol Scand. 2001; 59(3):167-73. DOI: 10.1080/000163501750266765. View

11.

Blobe G, Schiemann W, Lodish H . Role of transforming growth factor beta in human disease. N Engl J Med. 2000; 342(18):1350-8. DOI: 10.1056/NEJM200005043421807. View

12.

Mize T, Sundararaj K, Leite R, Huang Y . Increased and correlated expression of connective tissue growth factor and transforming growth factor beta 1 in surgically removed periodontal tissues with chronic periodontitis. J Periodontal Res. 2014; 50(3):315-9. PMC: 4289131. DOI: 10.1111/jre.12208. View

13.

Ohshima M, Yamaguchi Y, Matsumoto N, Micke P, Takenouchi Y, Nishida T . TGF-β signaling in gingival fibroblast-epithelial interaction. J Dent Res. 2010; 89(11):1315-21. DOI: 10.1177/0022034510378423. View

14.

Saifi B, Rezaee S, Tajik N, Erfanian Ahmadpour M, Ashrafi M, Vakili R . Th17 cells and related cytokines in unexplained recurrent spontaneous miscarriage at the implantation window. Reprod Biomed Online. 2014; 29(4):481-9. DOI: 10.1016/j.rbmo.2014.06.008. View

15.

Cheng W, Hughes F, Taams L . The presence, function and regulation of IL-17 and Th17 cells in periodontitis. J Clin Periodontol. 2014; 41(6):541-9. DOI: 10.1111/jcpe.12238. View

16.

Favre N, Bordmann G, Rudin W . Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR. J Immunol Methods. 1997; 204(1):57-66. DOI: 10.1016/s0022-1759(97)00033-1. View