Two GPSes in a Ball: Deciphering the Endosomal Tug-of-War Using Plasmonic Dark-Field STORM

Overview

Journal JACS Au

Publisher American Chemical Society

Specialty Chemistry

Date 2022 Aug 1

PMID 35911456

Authors

Siwoo Jin

Jiseong Park

Wonhee John Lee

Yongdeok Ahn

Youngchan Park

Minsoo Park

Inchan Hwang

Kwanyong Seo

Daeha Seo

Affiliations

Soon will be listed here.

Abstract

Live video recording of intracellular material transport is a promising means of deciphering the fascinating underlying mechanisms driving life at the molecular level. Such technology holds the key to realizing real-time observation at appropriate resolutions in three-dimensional (3D) space within living cells. Here, we report an optical microscopic method for probing endosomal dynamics with proper spatiotemporal resolution within 3D space in live cells: plasmonic dark-field STORM (pdf-STORM). We first confirmed that pdf-STORM has a spatial resolution comparable to that of scanning electron microscopy. Additionally, by observing two optical probes within a single organelle, we were able to track rotational movements and demonstrate the feasibility of using pdf-STORM to observe the angular displacements of an endosome during a "tug-of-war" over an extended period. Finally, we show various biophysical parameters of the hitherto unelucidated dynamics of endosomes-angular displacement is discontinuous and -axis movement predominates and follows a long-tail distribution.

Citing Articles

Temporal Patterns of Angular Displacement of Endosomes: Insights into Motor Protein Exchange Dynamics.

Jin S, Ahn Y, Park J, Park M, Lee S, Lee W Adv Sci (Weinh). 2024; 11(29):e2306849.

PMID: 38828676 PMC: 11304332. DOI: 10.1002/advs.202306849.

Single-Molecule Imaging of Membrane Proteins on Vascular Endothelial Cells.

Park J, Jin S, Jang J, Seo D J Lipid Atheroscler. 2023; 12(1):58-72.

PMID: 36761059 PMC: 9884557. DOI: 10.12997/jla.2023.12.1.58.

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