Identification and Quantification of Chlorogenic Acids from the Root Bark of by UHPLC-Q-Exactive Orbitrap Mass Spectrometry

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2022 Aug 1

PMID 35910160

Authors

Jianbo Yang

Lingwen Yao

Kaiyan Gong

Kailin Li

Lei Sun

Wei Cai

Affiliations

Soon will be listed here.

Abstract

The purpose of this study is to identify and quantify the chlorogenic acids (CGAs) from the root bark of , which is conventionally regarded as a tonic in folk Chinese Traditional medicine. The effective methods for identification and quantification analysis of CGAs were developed based on ultra high performance liquid chromatography-Q-exactive orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) in parallel reaction monitoring (PRM) and selected reaction monitoring (SIM), which showed high sensitivity and resolution for screening and quantifying compounds. The root bark of was extracted under ultrasonication with 70% methanol. Ultimately, a for total of 70 CGAs, 64 of these were tentatively identified for the first time. Moreover, a methodological study of seven kinds of CGAs was carried out. The proposed procedure was optimized and validated in terms of selectivity, linearity of analytical curves ( > 0.990), accuracy (recovery range from 96.7 to 105%), and repeatability (relative standard deviation <5%). Then it was applied to determine the content of the CGAs in roots from 66 of different batches. The total CGAs was quantified in a range between 2.150 and 33.51 mg/g, which could be considered as excellent source of natural bioactive compound. The result was extremely useful for understanding the bioactive substance and quality control of in depth.

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