Dehydroglyasperin D Suppresses Melanin Synthesis Through MITF Degradation in Melanocytes

Overview

Journal J Microbiol Biotechnol

Specialties Biotechnology
Microbiology

Date 2022 Jul 31

PMID 35909194

Authors

Eun Ji Baek

Yu-Bin Ha

Ji Hye Kim

Ki Won Lee

Soon Sung Lim

Nam Joo Kang

Affiliations

Soon will be listed here.

Abstract

Licorice () has been used as preventive and therapeutic material for hyperpigmentation disorders. Previously, we isolated noble compounds including dehydroglyasperin C (DGC), dehydroglyasperin D (DGD) and isoangustone A (IAA) from licorice hexane/ethanol extracts. However, their anti-melanogenic effects and underlying molecular mechanisms are unknown. The present study compared effects of DGC, DGD and IAA on pigmentation in melan-a melanocytes and human epidermal melanocytes (HEMn). DGD exerted the most excellent anti-melanogenic effect, followed by DGC and IAA at non-cytotoxic concentrations. In addition, DGD significantly inhibited tyrosinase activity in vitro cell-free system and cell system. Western blot result showed that DGD decreased expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein-1 (TRP-1) in melan-a cells and HEMn cells. DGD induced phosphorylation of MITF, ERK and Akt signal pathway promoting MITF degradation system. However, DGD did not influence p38 and cAMP-dependent protein kinase (PKA)/CREB signal pathway in melan-a cells. These result indicated that DGD inhibited melanogenesis not only direct regulation of tyrosinase but also modulating intracellular signaling related with MITF level. Collectively, these results suggested a protective role for DGD against melanogenesis.

Citing Articles

Inhibitory Effects of Polyphenol- and Flavonoid-Enriched Rice Seed Extract on Melanogenesis in Melan-a Cells via MAPK Signaling-Mediated MITF Downregulation.

Monmai C, Kim J, Chin J, Lee S, Baek S Int J Mol Sci. 2023; 24(14).

PMID: 37511600 PMC: 10380342. DOI: 10.3390/ijms241411841.

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