Spirotetramat Resistance in Myzus Persicae (Sulzer) (Hemiptera: Aphididae) and Its Association with the Presence of the A2666V Mutation

Overview

Journal Pest Manag Sci

Specialties Biology
Toxicology

Date 2022 Jul 28

PMID 35900771

Authors

Paul A Umina

Chris Bass

Anthony van Rooyen

Evatt Chirgwin

Aston L Arthur

Adam Pym

Jo Mackisack

Andrew Mathews

Lisa Kirkland

Affiliations

Soon will be listed here.

Abstract

Background: Chemicals are widely used to protect field crops against aphid pests and aphid-borne viral diseases. One such species is Myzus persicae (Sulzer), a global pest that attacks a broad array of agricultural crops and transmits many economically damaging plant viruses. This species has evolved resistance to a large number of insecticide compounds as a result of widespread and repeated chemical use in many parts of the world. In this study, we investigated the evolution of resistance to a new plant protection product, spirotetramat, following reported chemical control failures.

Results: Our study provides clear phenotypic and genotypic evidence of spirotetramat resistance in populations of M. persicae from Australia. We show there is cross-resistance to other insecticides within the same chemical group, namely spiromesifen and spirodiclofen. We also demonstrate that resistance is associated with the previously reported mutation, A2226V in the target site of spirotetramat, acetyl-CoA carboxylase. Our genetic analysis found all resistant M. persicae populations belong to the same multi-locus clonal type and carry the A2226V mutation, which appears to be inherited as a dominant trait in this species.

Conclusion: Our findings provide new insight into the resistance conferred by A2226V and have implications for the control of M. persicae in Australia and worldwide. A diagnostic assay developed in this study should serve as a valuable tool for future resistance monitoring and to support the implementation of pest management strategies. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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References

Blacket M, Robin C, Good R, Lee S, Miller A . Universal primers for fluorescent labelling of PCR fragments--an efficient and cost-effective approach to genotyping by fluorescence. Mol Ecol Resour. 2012; 12(3):456-63. DOI: 10.1111/j.1755-0998.2011.03104.x. View

Umina P, McDonald G, Maino J, Edwards O, Hoffmann A . Escalating insecticide resistance in Australian grain pests: contributing factors, industry trends and management opportunities. Pest Manag Sci. 2018; 75(6):1494-1506. DOI: 10.1002/ps.5285. View

Anstead J, Mallet J, Denholm I . Temporal and spatial incidence of alleles conferring knockdown resistance to pyrethroids in the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae), and their association with other insecticide resistance mechanisms. Bull Entomol Res. 2007; 97(3):243-52. DOI: 10.1017/S0007485307004889. View

Vorburger C, Lancaster M, Sunnucks P . Environmentally related patterns of reproductive modes in the aphid Myzus persicae and the predominance of two 'superclones' in Victoria, Australia. Mol Ecol. 2003; 12(12):3493-504. DOI: 10.1046/j.1365-294x.2003.01998.x. View

Jeschke P, Nauen R, Gutbrod O, Beck M, Matthiesen S, Haas M . Flupyradifurone (Sivanto™) and its novel butenolide pharmacophore: Structural considerations. Pestic Biochem Physiol. 2015; 121:31-8. DOI: 10.1016/j.pestbp.2014.10.011. View

Singh K, Cordeiro E, Troczka B, Pym A, Mackisack J, Mathers T . Global patterns in genomic diversity underpinning the evolution of insecticide resistance in the aphid crop pest Myzus persicae. Commun Biol. 2021; 4(1):847. PMC: 8263593. DOI: 10.1038/s42003-021-02373-x. View

Umina P, Edwards O, Carson P, van Rooyen A, Anderson A . High levels of resistance to carbamate and pyrethroid chemicals widespread in Australian Myzus persicae (Hemiptera: Aphididae) populations. J Econ Entomol. 2014; 107(4):1626-38. DOI: 10.1603/ec14063. View

Bass C, Puinean A, Zimmer C, Denholm I, Field L, Foster S . The evolution of insecticide resistance in the peach potato aphid, Myzus persicae. Insect Biochem Mol Biol. 2014; 51:41-51. DOI: 10.1016/j.ibmb.2014.05.003. View

Brownsey R, Boone A, Elliott J, Kulpa J, Lee W . Regulation of acetyl-CoA carboxylase. Biochem Soc Trans. 2006; 34(Pt 2):223-7. DOI: 10.1042/BST20060223. View

10.

Anstead J, Williamson M, Eleftherianos I, Denholm I . High-throughput detection of knockdown resistance in Myzus persicae using allelic discriminating quantitative PCR. Insect Biochem Mol Biol. 2004; 34(8):871-7. DOI: 10.1016/j.ibmb.2004.06.002. View

11.

Bass C, Puinean A, Andrews M, Cutler P, Daniels M, Elias J . Mutation of a nicotinic acetylcholine receptor β subunit is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae. BMC Neurosci. 2011; 12:51. PMC: 3121619. DOI: 10.1186/1471-2202-12-51. View

12.

Zamoum T, Simon J, Crochard D, Ballanger Y, Lapchin L, Vanlerberghe-Masutti F . Does insecticide resistance alone account for the low genetic variability of asexually reproducing populations of the peach-potato aphid Myzus persicae?. Heredity (Edinb). 2005; 94(6):630-9. DOI: 10.1038/sj.hdy.6800673. View

13.

Pym A, Umina P, Reidy-Crofts J, Troczka B, Matthews A, Gardner J . Overexpression of UDP-glucuronosyltransferase and cytochrome P450 enzymes confers resistance to sulfoxaflor in field populations of the aphid, Myzus persicae. Insect Biochem Mol Biol. 2022; 143:103743. DOI: 10.1016/j.ibmb.2022.103743. View

14.

Roy L, Fontaine S, Caddoux L, Micoud A, Simon J . Dramatic changes in the genotypic frequencies of target insecticide resistance in French populations of Myzus persicae (Hemiptera: Aphididae) over the last decade. J Econ Entomol. 2013; 106(4):1838-47. DOI: 10.1603/ec12475. View

15.

Lueke B, Douris V, Hopkinson J, Maiwald F, Hertlein G, Papapostolou K . Identification and functional characterization of a novel acetyl-CoA carboxylase mutation associated with ketoenol resistance in Bemisia tabaci. Pestic Biochem Physiol. 2020; 166:104583. DOI: 10.1016/j.pestbp.2020.104583. View

16.

Slater R, Paul V, Andrews M, Garbay M, Camblin P . Identifying the presence of neonicotinoidresistant peach-potato aphid (Myzus persicae) in the peach-growing regions of southern France and northern Spain. Pest Manag Sci. 2011; 68(4):634-8. DOI: 10.1002/ps.2307. View

17.

Puinean A, Elias J, Slater R, Warren A, Field L, Williamson M . Development of a high-throughput real-time PCR assay for the detection of the R81T mutation in the nicotinic acetylcholine receptor of neonicotinoid-resistant Myzus persicae. Pest Manag Sci. 2012; 69(2):195-9. DOI: 10.1002/ps.3300. View

18.

de Little S, Edwards O, van Rooyen A, Weeks A, Umina P . Discovery of metabolic resistance to neonicotinoids in green peach aphids (Myzus persicae) in Australia. Pest Manag Sci. 2016; 73(8):1611-1617. DOI: 10.1002/ps.4495. View

19.

Peng T, Pan Y, Yang C, Gao X, Xi J, Wu Y . Over-expression of CYP6A2 is associated with spirotetramat resistance and cross-resistance in the resistant strain of Aphis gossypii Glover. Pestic Biochem Physiol. 2016; 126:64-9. DOI: 10.1016/j.pestbp.2015.07.008. View

20.

Sloane M, Sunnucks P, Wilson A, Hales D . Microsatellite isolation, linkage group identification and determination of recombination frequency in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Genet Res. 2001; 77(3):251-60. DOI: 10.1017/s0016672301005018. View