Platelet-rich Plasma Promotes Peripheral Nerve Regeneration After Sciatic Nerve Injury

Overview

Journal Neural Regen Res

Date 2022 Jul 28

PMID 35900433

Authors

Su-Long Wang

Xi-Lin Liu

Zhi-Chen Kang

Yue-Shu Wang

Affiliations

Soon will be listed here.

Abstract

The effect of platelet-rich plasma on nerve regeneration remains controversial. In this study, we established a rabbit model of sciatic nerve small-gap defects with preserved epineurium and then filled the gaps with platelet-rich plasma. Twenty-eight rabbits were divided into the following groups (7 rabbits/group): model, low-concentration PRP (2.5-3.5-fold concentration of whole blood platelets), medium-concentration PRP (4.5-6.5-fold concentration of whole blood platelets), and high-concentration PRP (7.5-8.5-fold concentration of whole blood platelets). Electrophysiological and histomorphometrical assessments and proteomics analysis were used to evaluate regeneration of the sciatic nerve. Our results showed that platelet-rich plasma containing 4.5-6.5- and 7.5-8.5-fold concentrations of whole blood platelets promoted repair of sciatic nerve injury. Proteomics analysis was performed to investigate the possible mechanism by which platelet-rich plasma promoted nerve regeneration. Proteomics analysis showed that after sciatic nerve injury, platelet-rich plasma increased the expression of integrin subunit β-8 (ITGB8), which participates in angiogenesis, and differentially expressed proteins were mainly enriched in focal adhesion pathways. Additionally, two key proteins, ribosomal protein S27a (RSP27a) and ubiquilin 1 (UBQLN1), which were selected after protein-protein interaction analysis, are involved in the regulation of ubiquitin levels in vivo. These data suggest that platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury by affecting angiogenesis and intracellular ubiquitin levels.

Citing Articles

The efficacy of platelet-derived extracellular vesicles in the treatment of diabetic wounds: a systematic review and meta-analysis of animal studies.

Cai Z, Wang Y, Hu S, Yuan Q, Liu J, Luo C Arch Dermatol Res. 2025; 317(1):244.

PMID: 39812853 DOI: 10.1007/s00403-024-03781-9.

Platelet-rich plasma (PRP) in nerve repair.

Wang S, Liu Z, Wang J, Cheng L, Hu J, Tang J Regen Ther. 2024; 27:244-250.

PMID: 38586873 PMC: 10997806. DOI: 10.1016/j.reth.2024.03.017.

Reduced Graphene Oxide Fibers Combined with Electrical Stimulation Promote Peripheral Nerve Regeneration.

Zhao Y, Liu Y, Lu C, Sun D, Kang S, Wang X Int J Nanomedicine. 2024; 19:2341-2357.

PMID: 38469057 PMC: 10926921. DOI: 10.2147/IJN.S449160.

Platelet-Rich Plasma in Dermatology: New Insights on the Cellular Mechanism of Skin Repair and Regeneration.

Manole C, Soare C, Ceafalan L, Voiculescu V Life (Basel). 2024; 14(1).

PMID: 38255655 PMC: 10817627. DOI: 10.3390/life14010040.

Plasma exosomes improve peripheral neuropathy via miR-20b-3p/Stat3 in type I diabetic rats.

Li J, Wu G, Li W, Zhou X, Li W, Xu X J Nanobiotechnology. 2023; 21(1):447.

PMID: 38001489 PMC: 10675980. DOI: 10.1186/s12951-023-02222-5.

References

Khayer N, Mirzaie M, Marashi S, Jalessi M . Rps27a might act as a controller of microglia activation in triggering neurodegenerative diseases. PLoS One. 2020; 15(9):e0239219. PMC: 7498011. DOI: 10.1371/journal.pone.0239219. View

Xu Z, He Z, Shu L, Li X, Ma M, Ye C . Intra-Articular Platelet-Rich Plasma Combined With Hyaluronic Acid Injection for Knee Osteoarthritis Is Superior to Platelet-Rich Plasma or Hyaluronic Acid Alone in Inhibiting Inflammation and Improving Pain and Function. Arthroscopy. 2020; 37(3):903-915. DOI: 10.1016/j.arthro.2020.10.013. View

. Database Resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2016; 45(D1):D12-D17. PMC: 5210554. DOI: 10.1093/nar/gkw1071. View

Zuidema A, Wang W, Sonnenberg A . Crosstalk between Cell Adhesion Complexes in Regulation of Mechanotransduction. Bioessays. 2020; 42(11):e2000119. DOI: 10.1002/bies.202000119. View

Hiltunen M, Lu A, Thomas A, Romano D, Kim M, Jones P . Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion. J Biol Chem. 2006; 281(43):32240-53. DOI: 10.1074/jbc.M603106200. View

Li C, Liu S, Pi W, Zhang P . Cortical plasticity and nerve regeneration after peripheral nerve injury. Neural Regen Res. 2021; 16(8):1518-1523. PMC: 8323687. DOI: 10.4103/1673-5374.303008. View

Shen Y, Li T, Chou Y, Ho T, Ke M, Chen L . Comparison of perineural platelet-rich plasma and dextrose injections for moderate carpal tunnel syndrome: A prospective randomized, single-blind, head-to-head comparative trial. J Tissue Eng Regen Med. 2019; 13(11):2009-2017. DOI: 10.1002/term.2950. View

Qin J, Wang L, Zheng L, Zhou X, Zhang Y, Yang T . Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway. Int J Mol Med. 2016; 37(5):1363-70. DOI: 10.3892/ijmm.2016.2520. View

Qu W, Zhu Z, Liu J, Song D, Tian H, Chen B . Interaction between Schwann cells and other cells during repair of peripheral nerve injury. Neural Regen Res. 2020; 16(1):93-98. PMC: 7818858. DOI: 10.4103/1673-5374.286956. View

10.

Wang M, Chen G, Li G, Wang B, Lei C . Creating Cartilage in Tissue-Engineered Chamber Using Platelet-Rich Plasma Without Cell Culture. Tissue Eng Part C Methods. 2020; 26(7):375-383. DOI: 10.1089/ten.TEC.2020.0049. View

11.

Zheng C, Zhu Q, Liu X, Huang X, He C, Jiang L . Effect of platelet-rich plasma (PRP) concentration on proliferation, neurotrophic function and migration of Schwann cells in vitro. J Tissue Eng Regen Med. 2013; 10(5):428-36. DOI: 10.1002/term.1756. View

12.

Cheng H, Steinway M, Russell J, Feldman E . GTPases and phosphatidylinositol 3-kinase are critical for insulin-like growth factor-I-mediated Schwann cell motility. J Biol Chem. 2000; 275(35):27197-204. DOI: 10.1074/jbc.M002534200. View

13.

Garcia de Cortazar U, Padilla S, Lobato E, Delgado D, Sanchez M . Intraneural Platelet-Rich Plasma Injections for the Treatment of Radial Nerve Section: A Case Report. J Clin Med. 2018; 7(2). PMC: 5852429. DOI: 10.3390/jcm7020013. View

14.

Kurlawala Z, Dunaway R, Shah P, Gosney J, Siskind L, Ceresa B . Regulation of insulin-like growth factor receptors by Ubiquilin1. Biochem J. 2017; 474(24):4105-4118. PMC: 5842694. DOI: 10.1042/BCJ20170620. View

15.

Takalo M, Haapasalo A, Natunen T, Viswanathan J, Kurkinen K, Tanzi R . Targeting ubiquilin-1 in Alzheimer's disease. Expert Opin Ther Targets. 2013; 17(7):795-810. DOI: 10.1517/14728222.2013.791284. View

16.

Ucar O, Celik S . Comparison of platelet-rich plasma gel in the care of the pressure ulcers with the dressing with serum physiology in terms of healing process and dressing costs. Int Wound J. 2020; 17(3):831-841. PMC: 7948873. DOI: 10.1111/iwj.13344. View

17.

Moyle M, Napier M, McLean J . Cloning and expression of a divergent integrin subunit beta 8. J Biol Chem. 1991; 266(29):19650-8. View

18.

Chernousov M, Kaufman S, Stahl R, Rothblum K, Carey D . Alpha7beta1 integrin is a receptor for laminin-2 on Schwann cells. Glia. 2007; 55(11):1134-44. DOI: 10.1002/glia.20536. View

19.

ElSheshtawy A, Nazzal H, El Shahawy O, El Baz A, Ismail S, Kang J . The effect of platelet-rich plasma as a scaffold in regeneration/revitalization endodontics of immature permanent teeth assessed using 2-dimensional radiographs and cone beam computed tomography: a randomized controlled trial. Int Endod J. 2020; 53(7):905-921. DOI: 10.1111/iej.13303. View

20.

Qin J, Wang L, Sun Y, Sun X, Wen C, Shahmoradi M . Concentrated growth factor increases Schwann cell proliferation and neurotrophic factor secretion and promotes functional nerve recovery in vivo. Int J Mol Med. 2015; 37(2):493-500. DOI: 10.3892/ijmm.2015.2438. View