Metabolic Profiling of Species with Antitumor Activity

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Jul 28

PMID 35897874

Authors

Nerilson M Lima

Gagan Preet

Sara R Marqui

Thais de O R S Falcoski

Geovana Navegante

Christiane P Soares

Teresinha de Jesus A S Andrade

Felipe A La Porta

Harinantenaina Liva R Rakotondraie

Marcel Jaspars

Dulce H S Silva

Affiliations

Soon will be listed here.

Abstract

This work evaluated the metabolic profiling of species with antitumor potential. In addition, we described the antigenotoxicity of polyphenols isolated from and a proteomic approach using HepG2 cells after treatment with these metabolites. The in vitro cytotoxic activity against HepG2, HT-29 and T98G cancer cell lines was investigated. The assessment of genotoxic damage was carried out through the comet assay. The ethanolic extract from seeds was subjected to bioassay-guided fractionation and the most active fractions were characterized. One bioactive fraction with high cytotoxicity against HT-29 human colon cancer cells (IC = 4.0 µg mL) was found, and it was characterized as a mixture of -hydroxybenzoic acid and 4-vinyl-phenol. The fruit peel (IC = 18.6 µg mL) and seed (IC = 15.2 µg mL) extracts had cytotoxic activity against the cell line T98G, and its chemical composition showed a variety of phenolic acids. The chemical composition of this species indicated a wide variety of aromatic acids, flavonoids, tannins, and carotenoids. The high concentration (ranging from 5% to 30%) of these polyphenols in the bioactive extract may be responsible for the antitumor potential. Regarding the proteomic approach, we detected proteins directly related to the elimination of ROS, DNA repair, expression of tumor proteins, and apoptosis.

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