Developing Oligo Probes for Chromosomes Identification in Hemp ( L.)

Overview

Journal Plants (Basel)

Date 2022 Jul 27

PMID 35893604

Authors

Dmitry V Romanov

Gennady I Karlov

Mikhail G Divashuk

Affiliations

Soon will be listed here.

Abstract

Hemp ( L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.

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