Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2022 Jul 27

PMID 35891451

Authors

Jiahui He

Yukun Zhang

Zezhong Hu

Luxuan Zhang

Guanming Shao

Zi Xie

Yu Nie

Wenxue Li

Yajuan Li

Liyi Chen

Benli Huang

Fengsheng Chu

Keyu Feng

Wencheng Lin

Hongxin Li

Weiguo Chen

Xinheng Zhang

Qingmei Xie

Affiliations

Soon will be listed here.

Abstract

Waterfowl parvovirus (WPFs) has multiple effects on the intestinal tract, but the effects of recombinant Muscovy duck parvovirus (rMDPV) have not been elucidated. In this study, 48 one-day-old Muscovy ducklings were divided into an infected group and a control group. Plasma and ileal samples were collected from both groups at 2, 4, 6, and 8 days post-infection (dpi), both six ducklings at a time. Next, we analyzed the genomic sequence of the rMDPV strain. Results showed that the ileal villus structure was destroyed seriously at 4, 6, 8 dpi, and the expression of ZO-1, Occludin, and Claudin-1 decreased at 4, 6 dpi; 4, 6, 8 dpi; and 2, 6 dpi, respectively. Intestinal cytokines IFN-α, IL-1β and IL-6 increased at 6 dpi; 8 dpi; and 6, 8 dpi, respectively, whereas IL-2 decreased at 6, 8 dpi. The diversity of ileal flora increased significantly at 4 dpi and decreased at 8 dpi. The bacteria Ochrobactrum and Enterococcus increased and decreased at 4, 8 dpi; 2, 4 dpi, respectively. Plasma MDA increased at 2 dpi, SOD, CAT, and T-AOC decreased at 2, 4, 8 dpi; 4, 8 dpi; and 4, 6, 8 dpi, respectively. These results suggest that rMDPV infection led to early intestinal barrier dysfunction, inflammation, ileac microbiota disruption, and oxidative stress.

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