A Novel -Based Formulation Along with Mycorrhiza Inoculation for Controlling and Simultaneously Improving Growth, Nutrient Uptake, and Steviol Glycosides in Under Field Conditions

Overview

Journal Plants (Basel)

Date 2022 Jul 27

PMID 35890492

Authors

Jai Prakash

Dilfuza Egamberdieva

Naveen Kumar Arora

Affiliations

Soon will be listed here.

Abstract

The excess use of chemicals by farmers in the agroecosystems degrades soil quality, disturbs soil ecology, and increases soil salinity and health hazards in humans. is an important medicinal and aromatic crop whose leaves contain steviol glycosides (SGs). The NAIMCC-B-02323 strain STJP from the rhizosphere of producing salicylic acid (16.80 µg/mL), chitinase (75.58 U/mL), β-1,3-glucanase (220.36 U/mL), and cellulase (170 U/mL) was taken as a plant growth-promoting rhizobacteria (PGPR). The cell-free supernatant (CFS) from strain STJP showed significant biocontrol activity against (80%), suggesting the protective role of extracellular metabolite(s) against phytopathogens. Paneer whey-based bioformulation (P-WBF) was developed to exploit STJP to enhance the growth, nutrient uptake, soil properties, stevioside content, and SGs biosynthesis in under an -infested field. The combined treatment of P-WBF and mycorrhiza ( ABTEC) significantly enhanced plant growth parameters after 90 days, in comparison with control. The symbiotic action (P-WBF and mycorrhiza) displayed much better results in terms of chlorophyll a and b (improved by 132.85% and 39.80%, respectively), protein (by 278.75%), flavonoid (by 86.99%), carbohydrate (by 103.84%), antioxidant (by 75.11%), and stevioside (by 120.62%) contents in plants as compared to the untreated set. Further, the augmentation of potassium (by 132.39%), phosphorous (by 94.22%), and zinc (by 111.11%) uptake in plant tissues and soil was also observed by the application of P-WBF and mycorrhiza. The expression of UGT74G1 and UGT85C2 genes related to SG biosynthesis was upregulated (2.7- and 3.2-fold, respectively) in plants treated with P-WBF and mycorrhiza as further confirmed by the accumulation of SGs. The results suggest that the application of P-WBF and mycorrhiza not only provides an ecofriendly and sustainable solution to improve stevioside content in by a nutrient-linked mechanism but also paves the way to enhanced production of stevioside.

Citing Articles

Microbial fortification of pharmacological metabolites in medicinal plants.

Wu X, Yang Y, Zhang H Comput Struct Biotechnol J. 2023; 21:5066-5072.

PMID: 37867972 PMC: 10589376. DOI: 10.1016/j.csbj.2023.10.024.

References

Iatridis N, Kougioumtzi A, Vlataki K, Papadaki S, Magklara A . Anti-Cancer Properties of ; More than a Sweetener. Molecules. 2022; 27(4). PMC: 8874712. DOI: 10.3390/molecules27041362. View

Tewari S, Arora N . Multifunctional exopolysaccharides from Pseudomonas aeruginosa PF23 involved in plant growth stimulation, biocontrol and stress amelioration in sunflower under saline conditions. Curr Microbiol. 2014; 69(4):484-94. DOI: 10.1007/s00284-014-0612-x. View

Zheng J, Zhuang Y, Mao H, Jang I . Overexpression of SrDXS1 and SrKAH enhances steviol glycosides content in transgenic Stevia plants. BMC Plant Biol. 2019; 19(1):1. PMC: 6318952. DOI: 10.1186/s12870-018-1600-2. View

Ghosh S, Banerjee S, Pal S, Chakraborty N . Encountering epidemic effects of leaf spot disease (Alternaria brassicae) on Aloe vera by fungal biocontrol agents in agrifields-An ecofriendly approach. PLoS One. 2018; 13(3):e0193720. PMC: 5868775. DOI: 10.1371/journal.pone.0193720. View

Bennett A, Groten K . The Costs and Benefits of Plant-Arbuscular Mycorrhizal Fungal Interactions. Annu Rev Plant Biol. 2022; 73:649-672. DOI: 10.1146/annurev-arplant-102820-124504. View

Kang S, Radhakrishnan R, Lee I . Bacillus amyloliquefaciens subsp. plantarum GR53, a potent biocontrol agent resists Rhizoctonia disease on Chinese cabbage through hormonal and antioxidants regulation. World J Microbiol Biotechnol. 2015; 31(10):1517-27. DOI: 10.1007/s11274-015-1896-0. View

Prakash J, Arora N . Phosphate-solubilizing sp. enhances growth, phosphorus uptake and oil yield of L. 3 Biotech. 2019; 9(4):126. PMC: 6403274. DOI: 10.1007/s13205-019-1660-5. View

Behera B, Sethi B, Mishra R, Dutta S, Thatoi H . Microbial cellulases - Diversity & biotechnology with reference to mangrove environment: A review. J Genet Eng Biotechnol. 2019; 15(1):197-210. PMC: 6296582. DOI: 10.1016/j.jgeb.2016.12.001. View

Gawel-Beben K, Bujak T, Niziol-Lukaszewska Z, Antosiewicz B, Jakubczyk A, Karas M . Stevia rebaudiana Bert. leaf extracts as a multifunctional source of natural antioxidants. Molecules. 2015; 20(4):5468-86. PMC: 6272195. DOI: 10.3390/molecules20045468. View

10.

Meena M, Swapnil P, Upadhyay R . Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India. Sci Rep. 2017; 7(1):8777. PMC: 5562829. DOI: 10.1038/s41598-017-09138-9. View

11.

Sarmiento-Lopez L, Lopez-Meyer M, Sepulveda-Jimenez G, Cardenas L, Rodriguez-Monroy M . Photosynthetic performance and stevioside concentration are improved by the arbuscular mycorrhizal symbiosis in under different phosphate concentrations. PeerJ. 2020; 8:e10173. PMC: 7580585. DOI: 10.7717/peerj.10173. View

12.

Gao X, Guo H, Zhang Q, Guo H, Zhang L, Zhang C . Arbuscular mycorrhizal fungi (AMF) enhanced the growth, yield, fiber quality and phosphorus regulation in upland cotton (Gossypium hirsutum L.). Sci Rep. 2020; 10(1):2084. PMC: 7005850. DOI: 10.1038/s41598-020-59180-3. View

13.

Soria M, Audisio M . Inhibition of Bacillus cereus Strains by Antimicrobial Metabolites from Lactobacillus johnsonii CRL1647 and Enterococcus faecium SM21. Probiotics Antimicrob Proteins. 2014; 6(3-4):208-16. DOI: 10.1007/s12602-014-9169-z. View

14.

Ghawana S, Paul A, Kumar H, Kumar A, Singh H, Bhardwaj P . An RNA isolation system for plant tissues rich in secondary metabolites. BMC Res Notes. 2011; 4:85. PMC: 3079660. DOI: 10.1186/1756-0500-4-85. View

15.

Lee S, Shaari K . LC-MS metabolomics analysis of Stevia rebaudiana Bertoni leaves cultivated in Malaysia in relation to different developmental stages. Phytochem Anal. 2021; 33(2):249-261. DOI: 10.1002/pca.3084. View

16.

Azzam C, Al-Taweel S, Abdel-Aziz R, Rabea K, Abou-Sreea A, Rady M . Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Bertoni In Vitro. Plants (Basel). 2021; 10(4). PMC: 8074916. DOI: 10.3390/plants10040820. View

17.

Radonic A, Thulke S, Mackay I, Landt O, Siegert W, Nitsche A . Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun. 2004; 313(4):856-62. DOI: 10.1016/j.bbrc.2003.11.177. View

18.

Ahmad A, Ali H, Khan H, Begam A, Khan S, Ali S . Effect of Gibberellic Acid on Production of Biomass, Polyphenolics and Steviol Glycosides in Adventitious Root Cultures of (Bert.). Plants (Basel). 2020; 9(4). PMC: 7238111. DOI: 10.3390/plants9040420. View

19.

Nieves-Cordones M, Martinez V, Benito B, Rubio F . Comparison between Arabidopsis and Rice for Main Pathways of K(+) and Na(+) Uptake by Roots. Front Plant Sci. 2016; 7:992. PMC: 4932104. DOI: 10.3389/fpls.2016.00992. View

20.

Pickles B, Truong C, Watts-Williams S, Bueno C . Mycorrhizas for a sustainable world. New Phytol. 2020; 225(3):1065-1069. DOI: 10.1111/nph.16307. View